真核起始因子6调控转化生长因子β1对动脉粥样硬化的影响  

Effect of eIF6 regulating TGF-β1 on mice atherosclerosis

作　　者：杨帅 黄欣兴 孙雪梅 鲜雪梅 毕颖超 常佳佳 陈仁金 YANG Shuai;HUANG Xinxing;SUN Xuemei;XIAN Xuemei;BI Yingchao;CHANG Jiajia;CHEN Renjin(College of Life Sciences,Xuzhou Medical University,Xuzhou,Jiangsu 221004,China)

机构地区：[1]徐州医科大学生命科学学院,江苏徐州221004

出　　处：《现代医药卫生》2021年第10期1629-1631,共3页Journal of Modern Medicine & Health

基　　金：江苏省高等学校大学生创新创业训练计划(201810313045Y);徐州市社会发展项目(KC17090)。

摘　　要：目的探讨真核起始因子6(eIF6)调控转化生长因子β1(TGF-β1)对小鼠动脉粥样硬化的影响。方法将RAW264.7细胞分为磷酸盐缓冲液(PBS)组、脂多糖(LPS)组和白细胞介素4(IL-4)组,定量PCR测M1型巨噬细胞标志物诱导型一氧化氮合酶(iNOS)、单核细胞化学趋化蛋白(MCP-1)及M2型巨噬细胞标志物(CD163、Arg-1)及TGF-β1 mRNA表达。定量PCR和Western blotting检测TGF-β1在M1/M2型巨噬细胞中mRNA和蛋白的表达。在ApoE^(-/-)和ApoE^(-/-)/eIF6^(+/-)小鼠动脉粥样硬化模型,定量PCR检测小鼠腹主动脉中TGF-β1、IL-6、MCP-1和Arg-1 mRNA的表达。结果与PBS组比较,IL-4诱导后,细胞中CD163和Arg-1表达显著增加;LPS诱导后,细胞中iNOS和MCP-1表达显著增加。TGF-β1在M2型巨噬细胞中表达显著高于M1型巨噬细胞。eIF6慢病毒过表达转染细胞后,TGF-β1 mRNA在eIF6过表达细胞中表达水平显著下降(P<0.05)。ApoE^(-/-)/eIF6^(+/-)小鼠主动脉中TGF-β1和Arg-1 mRNA表达较ApoE^(-/-)小鼠显著升高;MCP-1和IL-6 mRNA表达显著降低。结论eIF6通过调节TGF-β1表达影响巨噬细胞极化,影响动脉粥样硬化发展。Objective To investigate effect of eIF6 regulating TGF-β1 on atherosclerosis in mice.Methods RAW264.7 cells were divided into PBS group,LPS group and IL-4 group.qPCR was used to detect the expression of M1 macrophage makers Inducible nitric oxide synthase(iNOS)and Monocyte chemotactic protein(MCP-1),M2 macrophage makers(CD163,Arg-1)and TGF-β1 mRNA.The expression of TGF-β1 mRNA and protein in M1/M2 macrophages were detected by qPCR and Western blotting.In ApoE^(-/-)and ApoE^(-/-)/eIF6^(+/-),the expression of TGF-β1,IL-6,MCP-1 and Arg-1 mRNA were detected by qPCR.Results Compared with PBS group,the expression of CD163 and Arg-1 in RAW264.7 cells increased significantly after IL-4 induction;after LPS induction,the expression of iNOS and MCP-1 increased significantly.The expression of TGF-β1 in M2 macrophages was significantly higher than that in M1 macrophages.RAW264.7 cells were transfected,TGF-β1 mRNA expression was significantly decreased.Compared with ApoE^(-/-),the expression of TGF-β1 and Arg-1 mRNA in ApoE^(-/-)/eIF6^(+/-)were significantly increased(P<0.05),while MCP-1 and IL-6 mRNA expression were significantly reduced.Conclusion eIF6 affects the polarization of macrophages by regulating the expression of TGF-β1 and affect the process of atherosclerosis.

关 键 词：真核起始因子6 转化生长因子Β1 巨噬细胞极化 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病]