葡萄籽原花青素对羔羊瘤胃发酵、血清炎症及抗氧化指标的影响  

Effects of High-Concentrate Diet Supplemented with Grape Seed Proanthocyanidins on Rumen fermentation,Inflammatory and Antioxidant Indicators of Rumen and Serum in Lambs

作　　者：郝小燕 牟春堂 乔栋 张暄梓 杨文军 赵俊星[1] 张春香[1] 张建新[1] HAO XiaoYan;MU ChunTang;QIAO Dong;ZHANG XuanZi;YANG WenJun;ZHAO JunXing;ZHANGChunXiang;ZHANG JianXin(College of Animal Science,Shanxi Agricultural University,Taigu 030801,Shanxi;Grassland Livestock Husbandry Development Center,Shuozhou 036002,Shanxi)

机构地区：[1]山西农业大学动物科学学院,山西太谷030801 [2]朔州市草牧业发展中心,山西朔州036002

出　　处：《中国农业科学》2021年第10期2239-2248,共10页Scientia Agricultura Sinica

基　　金：国家重点研发计划(2018YFD0502104);现代农业产业技术体系(CARS-38);山西省“1331工程”畜牧学重点学科建设计划(J201911301);山西省基础研究计划(201801D221291)。

摘　　要：【目的】研究高精料饲粮条件下,添加不同水平葡萄籽原花青素(GSPs)对杜寒杂交羔羊生长性能、瘤胃发酵、瘤胃及血清炎症因子及抗氧化能力的影响。为葡萄籽原花青素在反刍动物生产中的应用提供数据参考。【方法】选择48只体重相近(22.75±1.20 kg)、月龄相近的杜泊×小尾寒羊杂交公羔,采用单因素完全随机试验设计,将48只羔羊随机分为4组,每组12只。基础饲粮精粗比为7:3,GSPs添加量分别为0(对照组,CON)、10(10GSPs)、20(20GSPs)、40(40GSPs)mg·kg^(-1) BW。饲养期共60 d,前15 d为预饲期。正试期第1天晨饲前称量体重作为初始体重。正试期结束后颈静脉采集血液并分离血清,用于抗氧化指标、炎性因子和脂多糖含量测定;同时每组随机选择6只分别于饲喂后1、3、4、6、8、12 h口腔采集瘤胃液,立即测定瘤胃液pH,饲喂后3 h瘤胃液样品用于测定发酵指标及脂多糖含量。每组剩余6只进行屠宰,采集瘤胃组织测定抗氧化指标和炎症因子水平。【结果】10GSPs和20GSPs组的末体重显著高于对照组(P<0.05),与40GSPs组差异不显著(P>0.05)。10GSPs和20GSPs组ADG和ADFI显著高于对照组和40GSPs组(P<0.05),而40GSPs组与对照组差异不显著(P>0.05);添加GSPs对瘤胃pH有一定的调控作用,饲喂后3、8、12 h瘤胃pH随GSPs添加水平增加而线性升高(P<0.05),4 h瘤胃pH较对照组有线性升高趋势,但差异不显著(P=0.057);添加GSPs后瘤胃液中乙酸、丁酸、总挥发性脂肪酸浓度有降低的趋势(P<0.1),对丙酸、异戊酸、戊酸浓度及乙酸/丙酸值没有显著影响(P>0.05);添加GSPs后血清脂多糖浓度较对照组显著降低(P<0.05),但不影响瘤胃液中脂多糖浓度。20GSPs和40GSPs组瘤胃组织GSH-Px活性显著高于对照组和10GSPs组,MDA含量显著低于对照组和10GSPs组(P<0.05)。20GSPs和40GSPs组血清SOD活性显著高于对照组,GSH-Px活性显著高于对照组和10GSPs组(P<0.05)。添加GSPs对瘤胃�【Objective】This study was conducted to investigate the effects of dietary grape seed proanthocyanidins(GSPs)supplementation on growth performance,rumen fermentation,rumen and serum inflammatory factors and antioxidative activity in lambs under the condition of a high-concentrate diet,so as to provide a support for applying GSPs on ruminant.【Method】A total of forty-eight 1/2 Dorper×1/2 thin-tailed Han ram lambs with similar body weight(BW=22.75±1.20 kg,mean±SD)were randomly divided into four groups,with twelve lambs each group.Lambs were fed a 30:70 forage:concentrate diet with 0(control),10(10GSPs),20(20GSPs),and 40 mg·kg^(-1) BW(40GSPs)GSPs supplemented,respectively.The feeding experiment lasted for 60 days before sampling,with the first 15 days for diet transition and adaptation.On the first day of the formal experiment period,the BW before morning feeding was weighed as the initial BW.At the end of the trial period,blood samples were collected from the jugular vein,and the serum was separated for the determination of antioxidant and inflammatory indicators and lipopolysaccharide content.At the same time,six lambs in each group were randomly selected to collect rumen fluid by using an oral stomach tube at 1,3,4,6,8 and 12 hours after feeding,respectively,and the rumen pH were measured immediately.The fluid samples at 3 hours after feeding were used to measured fermentation parameters and lipopolysaccharide content.The other 6 lambs in each group were slaughtered,and the samples of rumen tissue were collected to determine the antioxidation index and inflammatory factors.【Result】The final weight of lambs in 10GSPs and 20GSPs groups were significant greater than that in control group(P<0.05),with no difference between 40GSPs and 10GSPs or 20GSPs(P>0.05).Lambs in 10GSPs and 20GSPs groups also had higher average daily gain and average daily feed intake than control and 40GSPs(P<0.05),with no significant difference between control and 40GSPs(P>0.05).The dietary GSPs supplementation had a certain regul

关 键 词：葡萄籽原花青素 羔羊 高精料 瘤胃发酵 抗氧化 

分 类 号：S826.5[农业科学—畜牧学]