内蒙古自治区牙克石市全沟硬蜱携带立克次体的研究  

Studying of Rickettsiae carried by Ixodes persulcatus in Yakeshi city,Inner Mongolia

作　　者：陆苗 覃新程[1] Lu Miao;Qin Xincheng(State Key Laboratory of Infectious Disease Prevention and Control,Department of Zoonoses,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,人兽共患病室,北京102206

出　　处：《疾病监测》2021年第3期276-279,共4页Disease Surveillance

基　　金：国家科技重大专项(No.2018ZX10712001-006-002)。

摘　　要：目的了解内蒙古自治区(内蒙古)大兴安岭地区蜱携带的斑点热群立克次体和无形体属细菌的流行情况与种类。方法在阿龙山林区采集蜱并根据形态学特征进行鉴定,运用PCR方法扩增17 kDa与16S rRNA基因分别对2010年捕获蜱中的两类病原体进行检测,同时扩增阳性样本中立克次体的全长16S rRNA和gltA基因,将扩增产物测序后进行序列分析。运用MegAlign软件分析序列的相似性,并通过PhyML3.1软件构建进化树进行系统发生分析。结果在阿龙山林区采集点共捕获蜱293只,经形态学鉴定全部为全沟硬蜱。其中斑点热群立克次体、无形体的感染率分别为9.56%和0。序列分析显示该地区蜱携带的斑点热群立克次体均为新塔拉塞维奇立克次体,16S rRNA和gltA基因与该种立克次体已知序列的同源性分别为100.00%和99.80%,氨基酸同源性为100.00%。进化分析结果显示本研究在牙克石市全沟硬蜱中检测到的新塔拉塞维奇立克次体与俄罗斯的菌株为同一基因型。结论内蒙古大兴安岭地区蜱中存在可致病的斑点热群新塔拉塞维奇立克次体。Objective To investigate the infection status of spotted fever group Rickettsiae and Anaplasma in ticks in the Greater Khingan Mountains area,Inner Mongolia.Methods Target genes of ticks collected in July,2010 were amplified by using PCR and the positive DNA were send to sequence.The nucleotide sequences similarity was calculated with software MegAlign.Phylogenetic analysis was performed by using software phyML3.1.Results We collected 293 ticks in Alongshan mountain area,which belonged to Ixodes persulcatus indicated by morphological identification.The infection rates of spotted fever group Rickettsiae and Anaplasma in the ticks were separately 9.56%and 0.Nucleotide sequence analysis confirmed that the spotted fever group Rickettsiae carried by the ticks was Candidatus Rickettsia tarasevichiae,the homology between the sequences obtained from this study and known reference sequences from GenBank was 100.00%for rrs gene,and 99.80%for gltA gene.The amino acid homology was 100.00%.Phylogenetic analysis indicated that the Candidatus Rickettsia tarasevichiae detected in this study in Ixodes persulcatus in Yakeshthis belonged to the same genotype of the strains detected in Russia.Conclusion The pathogen Candidatus Rickettsia tarasevichiae was carried by Ixodes persulcatus in the Greater Khingan Mountains area,Inner Mongolia.

关 键 词：斑点热群 蜱 进化分析 新塔拉塞维奇立克次体 

分 类 号：R211[医药卫生—中医学] R376