香烟烟雾颗粒通过ERK1/2信号通路上调肾上腺素α_(1)受体诱导血管平滑肌收缩  

Effect of cigarette smoking particles on the vasoconstrictions induced byα_(1)-adrenoceptor in the mesenteric artery in rats

作　　者：许梦荣 王川 路鹏鹏 李庆智 梁涛 常卓琳 徐仓宝 张恩户[1] XU Meng-rong;WANG Chuan;LU Peng-peng;LI Qing-zhi;LIANG Tao;CHANG Zhuolin;XU Cang-bao;ZHANG En-hu(College of Pharmacy,Shaanxi University of Chinese Medicine/Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang Shaanxi 712046;Shaanxi Provincial Key Laboratory of Ischemic Cardiovascular Diseases,Institute of Basic and Translational Medicine of Xi’an Medical University,Xi’an 710021)

机构地区：[1]陕西中医药大学药学院/陕西省中医药管理局中药药效机制与物质基础重点研究室,陕西咸阳712046 [2]西安医学院基础与转化医学研究所/陕西省缺血性心血管疾病重点实验室,西安710021

出　　处：《中南药学》2021年第5期831-835,共5页Central South Pharmacy

基　　金：国家自然科学基金(No.81800401,No.81470493);陕西中医药大学研究生创新项目(No.2020CX07);大学生创新创业训练计划项目(No.S202010716021)。

摘　　要：目的研究香烟烟雾颗粒对大鼠肠系膜动脉肾上腺素α_(1)受体的作用及机制。方法采用离体器官培养技术,离体肌张力描记观察苯肾上腺素引起的平滑肌收缩量效曲线变化,考察香烟烟雾诱导肾上腺素α_(1)受体在血管高反应性中的变化及调节肾上腺素α_(1)受体反应的相关信号通路。结果香烟烟雾可以增强肾上腺素α_(1)受体激动剂引起的血管收缩,ERK1/2通路抑制剂可减轻血管收缩,降低肾上腺素α_(1)受体的表达。NF-κB信号通路抑制剂和AMPK信号通路激活剂对香烟烟雾颗粒引起的肾上腺素α_(1)受体介导的血管收缩没有影响。结论香烟烟雾通过ERK1/2信号通路上调肾上腺素α_(1)受体介导的血管收缩。Objective To determine the effect of cigarette smoking particles on the vasoconstrictions induced byα_(1)-adrenoceptor in the mesenteric artery in rats.Methods In vitro organ culture was used to determine how cigarette smoke particles affected the vasoconstrictions induced byα_(1)-adrenoceptor and its mechanisms in vascular smooth muscle cells by myograph.Results Cigarette smoke particles enhanced the mesenteric artery constriction induced byα_(1)-adrenoceptor agonist.The inhibitors of ERK1/2 signaling pathway alleviated the vasocon striction and decreased the protein expression ofα_(1)-adrenoceptors.However,NF-κB signaling pathway inhibitors and AMPK signaling pathway activators had no effect on theα_(1)-adrenoceptor mediated vasoconstriction induced by cigarette smoke particles.Conclusion Cigarette smoking particles can enhance the vasoconstrictions mediated byα_(1)-adrenoceptor through ERK1/2 signaling pathway.

关 键 词：香烟烟雾颗粒 大鼠肠系膜动脉 肾上腺素α_(1)受体 ERK1/2信号通路 

分 类 号：R96[医药卫生—药理学]