人工智能气候室中灵芝富硒栽培研究  

Study on Selenium-enriched Cultivation of Ganodermal ucidum in the Artificial Intelligence Climate Chamber

作　　者：张玉华 张北壮[2] 黄仕祥 何焕清[3] 龚玉莲 曾碧健 蔡文韬 王璐 ZHANG Yuhua;ZHANG Beizhuang;HUANG Shixiang;HE Huanqing;GONG Yulian;ZENG Bijian;CAI Wentao;WANG Lu(Biology and Food Engineering Institute,Guangdong University of Education,Guangzhou 510303,China;School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China;Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)

机构地区：[1]广东第二师范学院生物与食品工程学院,广东广州510303 [2]中山大学生命科学学院,广东广州510275 [3]广东省农业科学院蔬菜研究所,广东广州510640

出　　处：《广东农业科学》2021年第4期8-13,共6页Guangdong Agricultural Sciences

基　　金：广东省教育厅青年创新人才项目(2018KQNCX182);广东省教育厅生态学特色重点学科建设项目。

摘　　要：【目的】在人工智能气候室中研究富硒灵芝的栽培方法,为灵芝商业化栽培和富硒产品的研究开发提供参考。【方法】以硒矿粉为硒源,以灵芝为富硒载体,采用固体基质栽培方式进行富硒灵芝培养,结合多糖和总三萜含量进行综合分析。【结果】6个试验处理的固体基质菌包分别添加0(CK)、1000、2000、3000、4000、5000μg硒,所产出的孢子粉硒含量分别为0.13、1.10、3.20、4.40、6.80、7.70μg/g,硒富集系数分别为1、8.5、24.6、33.8、52.3、59.2;所产出的子实体硒含量分别为0.35、1.30、5.15、10.90、16.25、17.05μg/g,硒富集系数分别为1、3.7、14.7、31.1、46.4、48.7。【结论】灵芝孢子粉和子实体中的硒含量随灵芝固体基质菌包中硒添加量的增加而提高。在6个试验处理中,灵芝孢子粉和子实体的产量以及所含多糖和总三萜的含量差异不显著。【Objective】The cultivation methods of selenium-enriched Ganoderma lucidum were studied in the artificial intelligence climate chamber in order to provide references for the commercial cultivation of G.lucidum and the research and development of selenium-enriched products.【Method】With selenium ore powder as selenium source and G.lucidum as selenium-enriched carrier,the selenium-enriched G.lucidum was cultivated by solid substrate cultivation method,and a comprehensive study was conducted combined with the polysaccharide and total triterpene contents.【Result】Among the six treatments,each solid substrate bacteria package was added with 0(CK),1000,2000,3000,4000 and 5000μg selenium,and the selenium contents in spore powder were 0.13,1.10,3.20,4.40,6.80 and 7.70μg/g,with the seenrichment coefficients of 1,8.5,24.6,33.8,52.3 and 59.2,respectively.The selenium contents of G.lucidum fruit body were 0.35,1.30,5.15,10.90,16.25 and 17.05μg/g,with the se-enrichment coefficients of 1,3.7,14.7,31.1,46.4 and 48.7,respectively.【Conclusion】There was positive correlation between the selenium contents in spore powder and fruit body,which increased with the increase of selenium in the solid substrate bacteria package.Among the six treatments,there was no significant difference in G.lucidum spore powder and fruit body production,as well as in polysaccharide and total triterpene contents.

关 键 词：富硒灵芝 灵芝孢子粉 硒源 人工智能气候室 

分 类 号：S567.31[农业科学—中草药栽培]