氧化还原介体强化厌氧活性污泥发酵产氢特征  

Improving fermentative hydrogen production of anaerobic sludge by redox mediators

作　　者：张立国[1,2] 艾冰凌 李建政 班巧英 ZHANG Li-guo;AI Bing-ling;LI Jian-zheng;BAN Qiao-ying(College of Environment and Resource Sciences,Shanxi University,Taiyuan 030006,China;Shanxi Laboratory for Yellow River,Taiyuan 030006,China;Haikou Experimental Station,Chinese Academy Tropical Agricultural Sciences,Haikou 571101,China;School of Environment,Harbin Institute of Technology,Harbin 150090,China)

机构地区：[1]山西大学环境与资源学院,山西太原030006 [2]山西省黄河实验室,山西太原030006 [3]中国热带农业科学院海口实验站,海南海口571101 [4]哈尔滨工业大学环境学院,黑龙江哈尔滨150090

出　　处：《中国环境科学》2021年第5期2196-2202,共7页China Environmental Science

基　　金：国家自然科学基金资助项目(51708341,51708548);哈尔滨工业大学城市水资源与水环境国家重点实验室开放基金项目(QA202137)。

摘　　要：为提高厌氧污泥的发酵产氢能力,采用间歇培养方式考察了氧化还原介体(ROMs)对厌氧污泥发酵葡萄糖产氢效能的影响,并通过Illumina MiSeq测序揭示了ROMs对微生物群落的影响.结果表明,在发酵液体积为100mL及葡萄糖初始浓度500mg/L条件下,对照的累计产氢量和最大产氢速率(R_(max))分别为11.0mL和0.28mL/h.当腐殖酸和蒽醌-2-磺酸钠(AQS)作为ROMs加入发酵体系后,厌氧污泥的产氢能力显著提高,其累计产氢量和R_(max)分别比对照高出56.4%、13.6%和53.6%、10.7%.相反,氧化石墨烯(GO)、指甲花醌和蒽醌-2,6-二磺酸钠(AQDS)导致厌氧污泥的产氢能力受到不同程度的抑制.Illumina MiSeq测序揭示了发酵系统的优势菌群存在显著差异.对照系统中的优势菌群主要来自5个属(Thermomarinilinea、Longilinea、Defluviimonas、Gimesia、Planctomicrobium),腐殖酸、AQS和指甲花醌发酵系统的主要优势菌群为Thermomarinilinea、Longilinea、Ottowia,而Thermomarinilinea、Longilinea、Ottowia、Defluviimonas为AQDS和GO发酵系统的主要菌群.冗余分析的结果表明,产氢量与Longilinea、Macellibacteroides、Trichococcus、Thioflavicoccus和Ottowia呈正相关,而与Defluviimonas、Pirellula、Gimesia和Ignavibacterium呈负相关.To improve the hydrogen production activity of anaerobic sludge,the effects of redox mediators(ROMs)on the hydrogen production efficiency of anaerobic sludge using glucose as substrate were investigated by batch culture.The microbial community structure was revealed by Illumina MiSeq sequencing.The results showed that the cumulative hydrogen production and maximum hydrogen production rate(R_(max))of the control were respectively 11.0mL and 0.28mL/h under conditions of the fermentative volume of 100mL and the initial glucose concentration of 500mg/L.When humic acid and anthraquinone-2-sulfonic acid(AQS)as ROMs were added to the system,the hydrogen production capacity of anaerobic sludge was significantly improved.The cumulative hydrogen production was higher than the control by 56.4%and 13.6%,respectively.While R_(max) was increased by 53.6%and 10.7%,respectively.On the contrary,the hydrogen production capacity of anaerobic sludge was inhibited when graphene oxide(GO),hennaquinone and anthraquinone-2,6-disulfonic acid(AQDS)were used as ROMs.Illumina MiSeq sequencing revealed that the dominant microbial groups from each sample were different.Thermomarinilinea,Longilinea,Defluviimonas,Gimesia and Planctomicrobium were the dominant genera in control.The major genera were shifted to Thermomarinilinea,Longilinea and Ottowia in humic acid,AQS and hennaquinone fermentation systems.While Thermomarinilinea,Longilinea,Ottowia and Defluviimonas became the predominant genera in AQDS and GO fermentation systems.Redundancy analysis indicated that hydrogen production was positively correlated with Longilinea,Macellibacteroides,Trichococcus,Thioflavicoccus and Ottowia,whereas negatively correlated with Defluviimonas,Pirellula,Gimesia and Ignavibacterium.

关 键 词：厌氧活性污泥 氧化还原介体 发酵产氢 微生物群落 

分 类 号：X703.5[环境科学与工程—环境工程]