磷化氢生物净化体系及抑制作用机理  

Inhibition mechanism of phosphine biological purification system

作　　者：余硕 刘树根[1] 李婷[1] YU Shuo;LIU Shu-gen;LI Ting(Faculty of Environmental Science and Engineering,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学环境科学与工程学院,云南昆明650500

出　　处：《中国环境科学》2021年第5期2219-2225,共7页China Environmental Science

基　　金：国家自然科学基金资助项目(51868029)。

摘　　要：在PH_(3)生物净化体系中添加呼吸链电子传递抑制剂,对比分析微生物生长代谢、磷的迁移转化、活性氧(ROS)产生及氧化酶活性等变化规律.当鱼藤酮或抗霉素A分别添加至R1、R2PH_(3)生物反应器后,其微生物体O_(2)^(-)·含量在运行时间14~17d期间的平均值分别上升至0.68,0.96mmol/g,明显高于空白对照组R0反应器.除自由基O_(2)^(-)·外,另一类型的活性氧HO·也存在于PH_(3)净化系统中;受累积的活性氧影响,生物体内丙二醛(MDA)含量处于较高水平,PH_(3)净化效率通常不超过75%,添加抑制剂的R1与R2反应体系在15~17d期间PH_(3)平均去除率分别下降至65.1%、59.5%.pH值、ROS含量以及氧化酶活性等因素均明显影响PH_(3)生物净化效果,当Fe^(3+)、Cu^(2+)、Mg^(2+)等金属阳离子迁移转运至微生物体内时,超氧化物歧化酶(SOD)与过氧化氢酶(CATase)活性得以不同程度的增强,可缓解活性物质O_(2)^(-)·或HO·导致的氧化胁迫作用,PH_(3)净化效能得以适度提升.Two electron transport inhibitors(rotenone and anti-mycin A)were introduced to phosphine bio-purification systems,then comparative experiments were conducted to investigate the variations of the microbial growth and metabolisms,phosphorus transport and transformation,the production of reactive oxygen species(ROS),and oxidase activities.When rotenone and anti-mycin A were respectively added to R1 and R2 bioreactors,the average O_(2)^(-)·content in these two systems increased to 0.68 and 0.96 mmol/g during the operation time of 14~17 days,which was significantly higher than control group(i.e.R0 reactor).Besides O_(2)^(-)·,another kind of reactive oxygen species,i.e.HO·,was also detected in bio-purification systems.In addition,due to the ROS accumulation,the malondialdehyde(MDA)content in microorganisms kept at a high level,caused the purification efficiency of phosphine less than 75%.The average phosphine removal efficiency in the R1 and R2 bioreactors decreased to 65.1%and 59.5%respectively,after 15~17 days operation.The factors,e.g.,pH,ROS,and oxidase activity,had significant effects on phosphine biological purification.As metal cations such as Mg^(2+)and Cu^(2+)migrated into the microbial cells,the enzyme activities of superoxide dismutase and catalase were dramatically improved,which led to the alleviation of the oxidative stress derived from the reactive species such as O_(2)^(-)·and HO·.As the consequence,the purification efficacy of phosphine was improved moderately.

关 键 词：PH_(3) 生物转化 抑制 活性氧 

分 类 号：X701[环境科学与工程—环境工程]