聚乳酸表面羧基化改性及细胞相容性研究  

Study on Surface Carboxylation Modification and Cytocompatibility of Poly(Lactic Acid)

作　　者：张博[1] 王小峰[1] 郭萌[1] 白志媛 任翠红 韩文娟[2] 宇山浩 李倩[1,2] ZHANG Bo;WANG Xiaofeng;GUO Meng;BAI Zhiyuan;REN Cuihong;HAN Wenjuan;UYAMA Hiroshi;LI Qian(Zhengzhou University,International Joint Research Laboratory for Micro-Nano Moulding Technology,School of Mechanics and Safety Engineering,Zhengzhou 450001,China;College of Materials Science and Engineering,Zhengzhou University,Zhengzhou 450001,China;Department of Applied Chemistry,Graduate School of Engineering,Osaka University,Suita 565-0871,Japan)

机构地区：[1]郑州大学力学与安全工程学院,微纳成型技术国家级国际联合研究中心,郑州450001 [2]郑州大学材料科学与工程学院,郑州450001 [3]大阪大学,日本大阪府吹田市565-0871

出　　处：《中国塑料》2021年第5期17-23,共7页China Plastics

基　　金：国际科技合作项目(2015DFA30550);河南省高等学校重点科技项目(19A430003);福建工程学院开放基金项目(KF-C19009)。

摘　　要：采用紫外活化二氧化氯自由基(ClO2·)氧化甲基的方法,在聚乳酸(PLA)表面接枝了羧基等含氧官能团,以增加PLA表面的亲水性和生物相容性。通过X射线光电子能谱分析和接触角分析,探究了改性后PLA表面元素组成的变化、最佳的氧化条件及氧化反应对PLA表面亲水性的影响。通过拉伸实验分析了氧化过程对PLA力学性能的影响。通过体外细胞增殖和细胞活性测试研究了改性后PLA表面的生物相容性变化。结果表明,羧基等含氧官能团在PLA表面成功生成;最佳氧化时间为10 min,最佳氧化温度为90℃,该条件下氧化后材料的表面接触角由82°降低至62.5°,表面亲水性得到改善;改性后PLA的拉伸强度不变,断裂伸长率略有下降;氧化后PLA表面显示出更好的细胞黏附能力和细胞增殖能力,更有利于细胞的生长。The oxygen-containing functional groups such as carboxyl groups were generated on the surface of poly(lactic acid)(PLA)by means of methyl oxidation through ultraviolet-activated sodium chlorite free radicals(ClO·).This can enhanced the hydrophilicity and cytocompatibility of the PLA surface.The surface element composition,optimum oxida-tion condition,and influence of oxidation reaction on the surface hydrophilicity of PLA were investigated by X-ray photo-electron spectroscopy and contact angle analysis.The effect of oxidation process on the mechanical properties of PLA was analyzed through a tensile test.The biocompatibility of the surface-modified PLA was studied by cell proliferation and cell activity tests in vitro.The experimental results indicated that the oxygen-containing functional groups such as car-boxyl group were successfully generated on the surface of PLA.The optimal oxidation time and temperature were deter-mined to be 10 min and 90℃,respectively.Under such a condition,the surface contact angle of the oxidized material de-creased from 82°to 62.5°,but its surface hydrophilicity was improved.The surface-modified PLA exhibited the un-changed tensile strength and a slight decrease in elongation at break.After oxidation,the surface of PLA showed better cell adhesion and cell proliferation,which was more beneficial to the growth of cells.

关 键 词：聚乳酸 表面改性 亲水性 细胞相容性 

分 类 号：TQ321[化学工程—合成树脂塑料工业]