局部微电流刺激对兔胫骨骨缺损部位降钙素基因相关肽表达的影响  

Effect of Local Microcurrent Stimulation on the Expression of Calcitonin Gene-Related Peptide in Tibial Defect of Rabbits

作　　者：姚玉英[1] 张宏[1] 范雅丽[1] 杨东浩[1] 金玉祥 贾丽丽[1] 郭志利[1] YAO Yuying;ZHANG Hong;FAN Yali;YANG Donghao;JIN Yuxiang;JIA Lili;GUO Zhili(Department of Pharmacology,Shijiazhuang Medical College,Shijiazhuang 050599,China)

机构地区：[1]石家庄医学高等专科学校解剖教研室,石家庄050599

出　　处：《中国中医骨伤科杂志》2021年第4期20-23,共4页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：河北省医学科学研究重点计划项目(20200159)。

摘　　要：目的:探讨局部微电流刺激对兔胫骨骨缺损部位降钙素基因相关肽(CGRP)表达的影响。方法:选择家兔40只,随机分为模型组和治疗组各20只,对两组家兔进行胫骨近端骨缺损造模,并对各组胫骨骨缺损部位骨密度(BMD)、骨矿物含量(BMC)及骨生物力学进行观察,免疫组化法对各组新生骨痂中CGRP及受体(CGRPR-1)表达情况进行检测。结果:造模1周后,两组家兔骨痂BMD及BMC差异无统计学意义(P>0.05),2、3及4周后,治疗组家兔的新生骨区BMD、BMC均高于模型组,差异有统计学意义(P<0.05)。实验4周后,治疗组家兔胫骨骨缺损部位的弹性载荷、弯曲能量、断裂载荷及刚性系数比模型组明显高,差异有统计学意义(P<0.05)。免疫组化结果显示,造模1周后,两组家兔新生骨组织CGRP及CGRPR-1表达差异无统计学意义(P>0.05),2、3及4周后,治疗组CGRP及CGRPR-1表达水平明显高于模型组,差异有统计学意义(P<0.05)。结论:局部微电流刺激可促进成骨细胞分化,加速骨矿物沉积,增加骨缺损部位的弯曲载荷强度,促进经肽分泌,加快胫骨骨缺损的愈合。Objective:To investigate the effect of local microcurrent stimulation on the expression of calcitonin gene-related peptide(CGRP) in the tibial defect of rabbits.Methods:40 rabbits were randomly divided into model group and treatment group with 20 rabbits in each.The model of proximal tibial bone defect was established in two groups of rabbits.Bone mineral density(BMD),bone mineral content(BMC) and biomechanics of tibial bone defect were observed in each group.The expression of CGRP and CGRPR-1 in each group was detected by immunohistochemistry.Results:There was no significant difference in callus BMD and BMC between the two groups at 1 week after the establishment of the model(P>0.05).While the BMD and BMC of the new bone area in the treatment group were higher than those in the model group after 2,3 and 4 weeks(P<0.05).The elastic load, bending energy, fracture load and rigidity coefficient of tibial defect in the treatment group were significantly higher than those in the model group after 4 weeks(P<0.05).The results of immunohistochemistry showed that there was no significant difference in the expression of CGRP and CGRPR-1 between the two groups at 1 week after the establishment of the model(P> 0.05),but both in the treatment group were significantly higher than those in the model group after 2,3 and 4 weeks(P<0.05).Conclusion:Local microcurrent stimulation can promote the differentiation of osteoblasts, accelerate the deposition of bone mineral, increase the bending load intensity of bone defect, promote the secretion of peptide, and accelerate the healing of tibial bone defect.

关 键 词：局部微电流刺激 胫骨 骨缺损 骨密度 降钙素基因相关肽 

分 类 号：R-33[医药卫生]