口蹄疫A型病毒不同毒株抗原反应性  

Comparison of antigen reactivity of different strains of foot-and-mouth disease virus serotype A

作　　者：孙燕燕[1,2] 陈夏辉 李昕 林密[1] 李峰松 贺华丽 包艳芳 杨光 蒋韬[1] SUN Yanyan;CHEN Xiahui;LI Xin;LIN Mi;LI Fengsong;HE Huali;BAO Yanfang;YANG Guang;JIANG Tao(State Reference Labratory of Food and Mouth Disease/State Key Laboratory of Animal Pathogen Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室/口蹄疫国家参考实验室,甘肃兰州730046 [2]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《中国兽医学报》2021年第3期451-456,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31772717);国家重点研发计划“猪重要疫病抗体快速检测技术研究”资助项目(2016YFD0500702-2)。

摘　　要：旨在对口蹄疫A型病毒(FMDV)3种毒株制备的不同抗原进行反应性比较分析,筛选最合适的免疫学诊断抗原,为进一步研究利用该抗原进行血清学诊断奠定基础。分别选择AF/72毒株、Re-A/WH/09毒株制备纯化146S抗原和VP1表达蛋白以及A/GD/MM/13毒株VP1表达蛋白作为备选抗原,利用间接ELISA检测方法与胶体金免疫层析检测方法(GICA),对100份背景清晰的田间样本血清进行平行检测,比较不同抗原的反应性。结果显示,A/GD/MM/13株抗原活性最低,间接ELISA方法检测AF/72株、Re-A/WH/09株纯化146S抗原和VP1表达蛋白对于A型阳性血清敏感性分别为90.00%、86.67%、78.33%和76.67%,与O型、Asia1型阳性血清和阴性血清特异性结果分别为95.00%、87.50%、75.00%和70.00%。GICA方法检测AF/72株、Re-A/WH/09株纯化146S抗原对于A型阳性血清敏感性分别为95.00%和83.33%,与O型、Asia1型阳性血清和阴性血清特异性结果分别为92.50%和87.50%。本研究通过FMDV-A型不同毒株抗原反应性的比较试验证实,AF/72株纯化146S抗原在间接ELISA和GICA 2种方法中均显示更好的敏感性、特异性,且在纯化过程中,收获量更多、浓度更高,为最合适的诊断用抗原,所建立的诊断方法可获得满意的检测结果。To compare and analyze the reactivity of different antigens prepared by three strains of foot-and-mouth disease virus type A(FMDV),and to choose the most suitable diagnostic antigens, so as to lay a foundation for the study of serological diagnosis.The purified 146 S protein of AF/72,VP1 expression protein of Re-A/WH/09,and VP1 expression protein of A/GD/MM/13 were selected as the candidate antigens.Indirect ELISA and GICA methods were established, and 100 serum samples with clear background were tested.The results showed that the antigen activity of A/GD/MM/13 strain was the lowest.Indirect ELISA revealed that the sensitivity of 146 S and VP1 expression protein purified from AF/72 and Re-A/WH/09 to FMDV type A positive serum was 90.00%,86.67%,78.33% and 76.67%,respectively.The specificity of them to the positive serum and the negative serum of type O and type Asia1 was 95.00%,87.50%,75.00% and 70.00%,respectively.In GICA,the sensitivity of 146 Spurified by AF/72 and Re-A/WH/09 was95.00%and 83.33%,respectively,and the specificity was 92.50%and 87.50%,respectively.In this study,the comparison of antigen reactivity of different strains of FMDV type A confirmed that146 Spurified by AF/72 showed better sensitivity and specificity in both indirect ELISA and GICA,and in the process of purification,the concentration of 146 Spurified by AF/72 is higher.As the most appropriate antigen,the diagnosis method established by using 146 Spurified by AF/72 can obtain a satisfactory result.

关 键 词：口蹄疫A型病毒 抗原反应性 间接ELISA 免疫层析法 

分 类 号：S852.65[农业科学—基础兽医学]