用HPLC法同时测定养血安神糖浆中4种成分的含量  

One step assay of the four plant ingredients in Yangxue Anshen syrup by HPLC method

作　　者：陆萍 倪东杰 郑巍 郭良君 王翔 LU Ping;NI Dongjie;ZHENG Wei;GUO Liangjun;WANG Xiang(Department of Pharmacy,72nd Group Army Hospital of PLA,Huzhou 313000,China)

机构地区：[1]陆军第七十二集团军医院,浙江湖州313000

出　　处：《药学实践杂志》2021年第3期270-273,共4页Journal of Pharmaceutical Practice

摘　　要：目的建立同时测定养血安神糖浆中槲皮苷、木犀草苷、芦丁和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的方法。方法采用HPLC法。色谱柱为Waters Symmetry C18(4.6 mm×250 mm,5μm)。流动相:0.1%醋酸(A)-甲醇(B)梯度洗脱,0~15 min,95%~90%A;15~35 min,90%~70%A;35~55 min,70%~60%A;55~85 min,60%~50%A;85~95 min,10%A。流速:1.0 ml/min,检测波长:256 nm和320 nm,柱温30℃,进样量10μl。结果槲皮苷、木犀草苷、芦丁和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷分别在10~300、5.0~150.0、5.0~150.0、20.0~600.0μg/ml范围呈良好的线性关系(r≥0.9989);加样回收率分别为(96.75±1.41)%、(99.61±1.01)%、(97.18±1.96)%和(99.12±0.97)%(n=6)。结论该方法简单、准确、稳定,可同时测定养血安神糖浆中4种成分的含量。Objective To establish a HPLC method for simultaneous determination of quercitrin,luteoloside,rutin and 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in Yangxue Anshen syrup.Methods Waters symmetry C18 column(250 mm×4.6 mm,5μm)was used with 0.1%acetic acid(A)and methanol(B)as the mobile phase.Gradient elution was performed at a flow rate of 1.0 ml/min,0-15 min,95%-90%A;15-35 min,90%-70%A;35-55 min,70%-60%A;55-85 min,60%-50%A;85-95 min,10%A.The detection wavelengths were 256 nm and 320 nm.Column temperature was 30℃and the injection volume was 10μl.Results Quercitrin,luteoloside,rutin and 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside showed good linear relationship within the range of 10-300,5.0-150.0,5.0-150.0,20.0-600.0μg/ml(r≥0.9989),respectively.The average recovery was(96.75±1.41)%,(99.61±1.01)%,(97.18±1.96)%and(99.12±0.97)%(n=6),respectively.Conclusion The established method is simple,accurate and stable,which can be used for the simultaneous determination of 4 components in Yangxue Anshen syrup.

关 键 词：养血安神糖浆 槲皮苷 木犀草苷 芦丁 2 3 5 4’-四羟基-二苯乙烯-2-O-β-D-葡萄糖苷 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]