盐度变化对金钱鱼感染嗜水气单胞菌后血清及肾脏免疫状态的影响  

Effects of salinity changes on serum and kidney immune status associated with injection of Aeromonas hydrophila in Scatophagus argus

作　　者：陆梦莹 苏冒亮 张俊彬[1,2] LU Mengying;SU Maoliang;ZHANG Junbin(National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;Shenzhen Key Laboratory of Marine Bioresource&Eco-Environmental Science,College of Life Sciences and Oceanography,Shenzhen University,Shenzhen 518060,China;Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,China)

机构地区：[1]上海海洋大学水产科学国家级实验教学示范中心,上海201306 [2]深圳市海洋生物资源与生态环境科学重点实验室,深圳大学生命科学与海洋学院,广东深圳518060 [3]光电子器件与系统教育部和广东省重点实验室,深圳大学光电工程学院,广东深圳518060

出　　处：《热带海洋学报》2021年第3期114-123,共10页Journal of Tropical Oceanography

基　　金：国家自然科学基金项目(41806177、41976108);中国博士后基金(2019M653010)。

摘　　要：季节性降雨和洋流运动常常使沿岸海水盐度呈现波动状态。环境盐度的改变往往导致鱼类更易受到病原的侵害而患病死亡。因此,探究盐度对金钱鱼(Sscatophagus argus)免疫功能的影响在渔业养殖业具有相当重要的参考价值。本文通过比较各盐度组金钱鱼经嗜水气单胞菌(Aeromonas hydrophila)感染后96h(96hpi)内血清和肾脏抗氧化酶(superoxide dismutase,SOD)浓度以及免疫相关参数Complement 4(C4)、Interleukin-6(IL-6)和Immunoglobulin M(IgM)的变化,来分析盐度的改变对其血清和肾脏免疫功能的影响。结果显示,除96hpi外,血液和肾脏组织中淡水组和低盐组中金钱鱼体内SOD浓度总体均比25‰盐度组高,且最大差异可达150ng·mL^(-1)左右,其中血清结果差异极显著(P<0.01);淡水组与低盐组中金钱鱼经嗜水气单胞菌感染后96h内血清和肾脏组织C4浓度总体均比25‰盐度组高100~600μg·mL^(-1) (P<0.01)左右;虽然三个盐度感染嗜水气单胞菌后组肾脏组织IL-6浓度(14.2±0.1、17.9±0.0和17.9±0.0pg·mL^(-1))显著低于对照组(P<0.01),25‰盐度组肾脏组织IL-6水平仍显著高于淡水组和低盐组(P<0.01),而淡水组的血清IL-6浓度在96hpi内显著增加至56.9±1.0pg·mL^(-1)(P<0.01);25‰盐度组金钱鱼血清和肾脏组织IgM浓度(71.8±2.9和6.3±0.4μg·mL^(-1))比淡水组和低盐组高约1~20μg·mL^(-1),且差异显著(P<0.05)。同时,25‰盐度组血清和肾脏组织大量产生抗体IgM时间(12hpi)早于淡水组和低盐组(24hpi)。综上所述, 25‰盐度组在细菌感染后受到的损伤较小,肾脏及血清免疫状态优于淡水组与低盐组,且免疫应答反应更迅速。因此推测,盐度的降低将会导致金钱鱼血清和肾脏免疫状态下降。本研究可为调节金钱鱼的养殖盐度提供参考。The salinity in coastal waters fluctuates frequently due to seasonal rainfall and ocean currents.Salinity change makes fish more susceptible to pathogens,leading to disease and death.As the main lymphoid tissue of teleosts,kidney is closely related to fish’s immune function.This study aims to explore effects of environmental salinity on the kidney immune function of Scatophagus argus.In order to analyze the relationship between the change of environmental salinity and immune situation in fish’s kidney after Aeromonas hydrophila injection,we compared the changes of superoxide dismutase(SOD)concentration in serum and kidney,and immunerelated parameters Complement 4(C4),Interleukin6(IL-6),and Immunoglobulin M(IgM)concentrations in kidney before and after infection within 96 h of each salinity group.Our results show that except for 96 hpi,SOD concentrations in the serum and kidney tissues of the freshwater group and lowsalinity(?‰)group were higher than those of the 25‰salinity group(P<0.01),and that the maximum difference between lowsalinity group and 25‰salinity group was about 150 ng·mL^(-1).The concentration of C4 in the serum and kidney of the freshwater group and lowsalinity group was about 100600μg·mL^(-1) higher than that of the 25‰salinity group within 96 hpi(P<0.01).The concentration of IL6 in the kidney tissues of S.argus in the 25‰salinity group was higher than that in the freshwater group and lowsalinity group(P<0.01),although IL6 concentrations in kidney of the three groups were significantly lower than that of the control group(14.2±0.1 pg·mL^(-1),in freshwater;17.9±0.0 pg·mL^(-1),in lowsalinity;17.9±0.0 pg·mL^(-1),in 25‰salinity)at 96 hpi(P<0.01),while the serum IL6 concentration in the freshwater group was significantly increased to 56.9±1.0 pg·mL^(-1)(P<0.01).The serum and kidney IgM levels of fish in the 25‰salinity grop(71.8±2.9μg·mL^(-1) and 6.3±0.4μg·mL^(-1))were about 120 g·mL^(-1) higher than those of the freshwater group and lowsalinity group(P<0.05).The ti

关 键 词：金钱鱼 盐度 嗜水气单胞菌 免疫 肾脏 

分 类 号：S943[农业科学—水产养殖]