白首乌中抗肿瘤活性成分告达庭-3-O-β-D-磁麻糖苷在大鼠体内的代谢产物分析  

Studies on the Metabolites of Caudatin-3-O-β-D-Cymaropyranoside in Rats

作　　者：施建丰 彭子萱[3] 彭蕴茹 姚孝明[2] 陈云[1] SHI Jian-feng;PENG Zi-xuan;PENG Yun-ru;YAO Xiao-ming;CHEN Yun(Department of Immunology, Nanjing Medical University, Nanjing, 211166, China;Department of Laboratory, Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing, 210028, China;The Third Clinical Medical College, Xinjiang Medical University, Urumqi, 830011, China;Department of Pharmacology and Toxicology, Jiangsu Provincial Institute of Traditional Chinese Medicine, Nanjing, 210028, China)

机构地区：[1]南京医科大学免疫学系,江苏南京211166 [2]江苏省中西医结合医院检验科,江苏南京210028 [3]新疆医科大学第三临床医学院,新疆乌鲁木齐830011 [4]江苏省中医药研究院药理毒理学研究室,江苏南京210028

出　　处：《南京中医药大学学报》2021年第3期428-433,共6页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81102884);江苏省自然科学基金(BK2008488)。

摘　　要：目的研究白首乌中甾苷成分告达庭-3-O-β-D-磁麻糖苷在大鼠体内的代谢情况。方法大鼠单剂量灌胃给予20 mg/kg的告达庭-3-O-β-D-磁麻糖苷,分别收集24 h内的血浆、尿液、粪便和胆汁,样品经处理后,采用UPLC-Q-TOF分析,结合MetabolynxTM软件初步鉴定告达庭-3-O-β-D-磁麻糖苷在大鼠血浆、尿液、粪便和胆汁中的代谢产物。结果在血浆、尿液、粪便和胆汁样品中发现了告达庭-3-O-β-D-磁麻糖苷的原型、水解及去甲基化代谢物,其中尿液中有原型,血浆和胆汁中有水解产物,粪便中有去2个甲基化产物,胆汁中有去1个和去3个甲基化产物。结论告达庭-3-O-β-D-磁麻糖苷在大鼠体内可发生较为广泛的代谢,其中主要代谢类型有水解和去甲基化。OBJECTIVE To study the rats'metabolism of caudatin-3-O-β-D-cymarose glycosides,a kind of sterioside in Radix Cynanchi Auriculati.METHODS Rats were given the a single dose of caudatin-3-O-β-D-cymarose glycosides by intragastric administration(20 mg/kg).Then the rats'plasma,urine,feces,and bile during the following 24 hours were collected.In addition,the processed samples were analyzed by UPLC-Q-TOF with MetabolynxTM software for the preliminary measurement of the metabolites of caudatin-3-O-β-D-cymarose glycosides in rats'plasma,urine,feces,and bile.RESULTS The original,hydrolytic and demethylated metabolites of caudatin-3-O-β-D-cymarose glycosides were found in plasma,urine,feces,and bile samples,specifically,the original caudatin-3-O-β-D-cymarose glycosides in urine,hydrolytic metabolites in plasma and bile,two methylated metabolites in feces,along with one methylated and three demethylated metabolites in bile.CONCLUSION The metabolism of caudatin-3-O-β-D-cymarose glycosides in rats is extensive,and the main types of metabolism are hydrolysis and demethylation.

关 键 词：白首乌 告达庭-3-O-β-D-磁麻糖苷 C21甾苷 代谢 

分 类 号：R284.1[医药卫生—中药学]