绿豆萌发期耐旱种质资源筛选评价和遗传多样性分析  

Genetic Diversity of Mung Bean Germplasms with Drought-tolerance at Seed Germination Stage

作　　者：黄年英 朱珍珍 刘昌燕[2] 廖芳丽 李莉[2] 陈宏伟[2] 刘良军[2] 韩雪松 万正煌[2] 沙爱华 HUANG Nianying;ZHU Zhenzhen;LIU Changyan;LIAO Fangli;LI Li;CHEN Hongwei;LIU Liangjun;HAN Xuesong;WAN Zhenghuang;SHA Aihua(College of Agriculture/Hubei Collaborative Innovation Center for Grain Industry,Yangtze University,Jingzhou,Hubei434025,China;Institute of Food Crops,Hubei Academy of Agricultural Sciences/Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement,Wuhan430064,China;Seed Authority of Jingzhou,Jingzhou,Hubei434020,China)

机构地区：[1]长江大学农学院/主要粮食作物产业化湖北省协同创新中心,湖北荆州434025 [2]湖北省农业科学院粮食作物研究所/粮食作物种质创新与遗传改良湖北省重点实验室,武汉430064 [3]荆州市种子管理局,湖北荆州434020

出　　处：《福建农业学报》2021年第3期255-263,共9页Fujian Journal of Agricultural Sciences

基　　金：国家重点研发计划(2019YFD1001304/2019YFD1001300);国家现代农业产业技术体系建设项目(CARS-09);湖北省技术创新重大专项(2018ABA090)。

摘　　要：【目的】鉴定56份绿豆种质种子萌发期耐旱性,明确其遗传特性。【方法】采用10%甘露溶液模拟干旱胁迫,以相对发芽率、相对发芽势和相对根长等10个指标作为耐旱性评价指标,通过主成分分析、隶属函数分析和多元分析方法鉴定种质耐旱性。同时基于SSR扩增对56份绿豆种质资源进行分子聚类,明确其遗传基础。【结果】干旱胁迫对萌发期10个耐旱性评价指标具有不同程度影响。主成分分析显示前三个综合指标贡献率分别为57.87%、16.42%、和11.42%,累积贡献率达到85.71%,可以代表10个评价指标的变异信息。根据主成分分析计算得到的综合评价值D对56份种质耐旱性进行评价,鉴定出2份高耐、10份耐旱、30份中耐、13份敏感和1份高敏种质。基于SSR聚类将56份种质划分为3个主要类群,第1类群包含25份种质,表现为较耐旱。第2类群包含26份种质,遗传基础复杂,表现出不同程度的耐旱性。第三类群包含5份种质,表现为偏敏感。【结论】明确了56份绿豆种质萌发期的耐旱性及其遗传特性,为利用这些种质开展绿豆耐旱育种奠定了基础。【Objective】Drought-tolerance at seed germination stage and genetic diversity of 56 mung bean varieties were determined for resource evaluation.【Method】Selected mung bean seeds were treated with either water as control or 10%mannitol solution to simulate drought stress.Ten indicators on the draught-tolerance of the seeds at germination stage,such as relative germination rate and potential as well as root length of the sprouts,were monitored.The results were compared to categorize the germplasms using principal component,membership function,and multivariate analyses.Then,the amplified SSRs of the categorized germplasms provided the genetic basis for classifying the draught-resistant property of the seeds at germination stage.【Result】The top 3 indicators of draught-tolerance comprised 57.87%,16.42%,and 11.42%separately,and 85.71%accumulatively,in contrition from a principal component analysis.And,at the calculated comprehensive evaluation value D,two of the 56 germplasms were graded highly tolerant,10 tolerant,30 moderately tolerant,13 susceptible,and 1 highly susceptible to drought.From the cluster analysis on the SSR data,the germplasms were classified into 25 of relatively drought-resistant,26 of varying degrees of drought-resistant due to complex genetic properties,and 5 of droughtsusceptible.【Conclusions】The drought-tolerance of the 56 mung bean germplasms at seed germination stage and their genetic characteristics were determined and classified to facilitate future breeding and utilization of the resource.

关 键 词：绿豆 耐旱 萌发期 隶属函数法 综合评价 遗传多样性分析 

分 类 号：S522[农业科学—作物学]