RNA测序探讨COPD患者外周血单个核细胞环状RNA表达差异  

RNA sequencing to investigate the difference of circular RNA expression in peripheral blood mononuclear cells of patients with COPD

作　　者：张君 詹峰[1,2] 孙娟 丁毅鹏[1,3] ZHANG Jun;ZHAN Feng;SUN Juan;DING Yi-peng(Hainan Hospital Affiliated to Hainan Medical College,Haikou 570311,China;Department of Emergency,Hainan Provincial People’s Hospital,Haikou 570311,China;Department of General Medicine,Hainan Provincial People's Hospital,Haikou 570311,China)

机构地区：[1]海南医学院附属海南医院,海南海口570311 [2]海南省人民医院急诊科,海南海口570311 [3]海南省人民医院全科医学科,海南海口570311

出　　处：《海南医学院学报》2021年第10期775-780,共6页Journal of Hainan Medical University

基　　金：国家自然科学基金资助项目(81160008、81660013)。

摘　　要：目的:通过RNA测序技术检测慢性阻塞性肺疾病(COPD)差异环状RNA并阐述其可能的功能与调控机制。方法:分别采集5例COPD患者与5名正常人外周血,分离单个核细胞,进行去线性化得到环状RNA,并建库测序,以变化倍数Foldchange绝对值≥2且P<0.05统计分析差异环状RNA,并通过来源基因功能与信号通路聚类分析环状RNA可能的功能与通路。结果:测序鉴定到1 215个环状RNA,具有显著差异的环状RNA共计187个,其中上调107个,下调80个;环状RNA来源基因功能富集最明显的功能是:细胞过程、细胞与结合。环状RNA来源基因变化最明显的前3个通路分别是:非同源末端连接通路、铁死亡通路与FoxO信号通路。结论:RNA测序发现了COPD中显著变化的环状RNA,以及环状RNA可能调控的功能与通路,为从环状RNA这个新角度理解COPD的发病机制提供理论依据。Objective:To detect COPD differential circular RNA and explain its possible functions and regulatory mechanisms using RNA sequencing technology.Methods:This study has collected the peripheral blood from 5 COPD patients and 5 normal people,separated mononuclear cells,de-linearized to obtain circular RNA,and constructed a library for sequencing.The difference is statistically analyzed with the Foldchange absolute value≥2 and P<0.05,and conducted cluster analysis of the possible functions and pathways of circular RNA by host gene function and signal pathway.Results:Sequencing identified 1215 circular RNAs and a total of 187 circular RNAs with significant differences,of which 107 were up-regulated and 80 were down-regulated.The most obvious functions of host genes from circular RNA enrichment are cell process,cell and binding.The first three pathways with the most obvious changes are non-homologous end junction pathway,iron death pathway,and FoxO signaling pathway.Conclusion:RNA sequencing has discovered the significantly changed circular RNA in COPD,as well as the functions and pathways that circular RNA may regulate,providing a theoretical basis for understanding the pathogenesis of COPD from a new perspective of circular RNA.

关 键 词：COPD 测序 环状RNA 机制 

分 类 号：R563.9[医药卫生—呼吸系统]