五味子乙素降低PM2.5抑制HTR细胞增殖的作用研究  

Schisandrin B Decreases the Effect of PM2.5 on HTR Cell Proliferation

作　　者：李晓 郭艳丽 韩俊婷 邹晓萍 董渠龙 LI Xiao;GUO Yan-li;HAN Jun-ting;ZOU Xiao-ping;DONG Qu-long(Department of Obstetrics and Gynecology,The Characteristic Medicine Center of the Chinese People′s Armed Police Forces,Tianjin 300162,China;Department of Reproductive Medicine,The Characteristic Medicine Center of the Chinese People′s Armed Police Forces,Tianjin 300162,China)

机构地区：[1]中国人民武装警察部队特色医学中心妇产科,天津300162 [2]中国人民武装警察部队特色医学中心生殖中心,天津300162

出　　处：《国际生殖健康／计划生育杂志》2021年第3期177-181,共5页Journal of International Reproductive Health/Family Planning

摘　　要：目的:探索五味子乙素(Schisandrin B,Sch B)是否可以降低PM2.5对人早孕胎盘绒毛膜外滋养层(human trophoblast HTR-8/SVneo,HTR)细胞增殖的抑制作用。方法:体外实验以HTR细胞为载体,利用MTS细胞增殖实验检测不同浓度的细颗粒物(PM2.5)、Sch B单独给药以及PM2.5联合Sch B给药对HTR细胞增殖的影响。结果:10~1000μg/mL浓度范围内的PM2.5均抑制了HTR细胞的增殖(P<0.01),其中150μg/mL浓度的PM2.5抑制率达到21.97%;0.1~5μmol/L浓度Sch B均降低了PM2.5的抑制HTR细胞增殖作用,且在0.1~2μmol/L浓度范围内其降低PM2.5抑制HTR细胞增殖的作用随着Sch B浓度增加而增大(P<0.01),其中2μmol/L的Sch B对HTR细胞的保护作用最强,保护率达到14.07%。结论:Sch B可以有效降低PM2.5对HTR细胞增殖的抑制作用,具有体内潜在保护人类滋养细胞的作用。Objective:To investigate whether Schisandrin B(Sch B)can attenuate the effect of PM2.5 on the proliferation of human trophoblast HTR-8/Svneo(HTR)cells.Methods:The in vitro cultured HTR cells were treated by PM2.5 of 10-1000μg/mL concentration,Sch B-alone and Sch B plus PM2.5.The proliferation of HTR cells was tested by MTS method.Results:PM2.5 of different concentrations from 10 to 1000μg/mL inhibited the proliferation of HTR cells(P<0.01),and the inhibition rate of 150μg/mL PM2.5 was 21.97%.Sch B of the concentration from 0.1 to 5μmol/L attenuated the PM2.5-induced inhibition of HTR cells proliferation,with a dose-effect relationship among 0.1-2μmol/L.Sch B of 2μmol/L presented the highest protection rate(14.07%).Conclusions:Sch B could attenuate the PM2.5-induced inhibition of HTR cell proliferation,suggesting a potential protection of human trophoblast cells in vivo.

关 键 词：五味子素 PM2.5 滋养层 细胞增殖 细胞培养技术 

分 类 号：R285[医药卫生—中药学]