声表面波气相色谱仪快速定性分析姜黄挥发油  

Rapid qualitative analysis of turmeric volatile oil by surface acoustic wave gas chromatograph

作　　者：柳小莉 朱宏伟[2] 王芳[3] 张小飞[4] 黄小英[3] 李慧婷 陆艳艳[2] 孙林 杨明[1,3] 何世堂[2] LIU Xiaoli;ZHU Hongwei;WANG Fang;ZHANG Xiaofei;HUANG Xiaoying;LI Huiting;LU Yanyan;SUN Lin;YANG Ming;HE Shitang(Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Institute of Acoustics,Chinese Academy of Sciences,Beijing 100190,China;Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;Shaanxi University of Chinese Medicine,Xianyang 712000,China)

机构地区：[1]成都中医药大学,成都611137 [2]中国科学院声学研究所,北京100190 [3]江西中医药大学,南昌330004 [4]陕西中医药大学,咸阳712000

出　　处：《应用声学》2021年第3期358-366,共9页Journal of Applied Acoustics

基　　金：国家自然科学基金项目(81703720);江西省重大科技研发专项(20194ABC28009)。

摘　　要：应用声表面波气相色谱仪快速定性分析姜黄挥发油。声表面波气相色谱仪采用DB-5毛细管色谱柱,初始温度45℃,按6◦C/s程序升温至160℃,将检测结果与气相色谱-质谱仪结果进行比较。在姜黄挥发油定性分析中,声表面波气相色谱仪20 s完成检测,而气相色谱-质谱仪需要30 min完成,声表面波气相色谱仪检测更高效;同时,声表面波气相色谱仪与气相色谱-质谱仪共同检测出姜黄挥发油中10种化学成分,且10种化学成分相对百分含量总和占挥发油总成分的90%以上,由于色谱柱长度区别,声表面波气相色谱仪检出的化学成分相对较少;定量分析中,对声表面波气相色谱仪进样方式进行了考察,顶空进样与液体进样对化学成分相对百分含量检测有较大差异;采用与气相色谱-质谱仪一致的液体进样方式,对姜黄挥发油中反式石竹烯定量分析,声表面波气相色谱仪最低检出限可以达到16.9 pg,在0.001624~0.01624 mg/mL范围内R=0:9976具有较好的线性关系,且精密度和重复性较好。声表面波气相色谱仪能够用于姜黄挥发油的快速定性和定量分析,为中药挥发油的分析提供了新的快捷的检测技术基础。The turmeric volatile oil was analyzed by surface acoustic wave gas chromatography(GC-SAW).The GC-SAW was carried out on a DB-5 capillary column with an initial temperature of 45℃ and a programmed temperature of 6◦C/s to 160℃.The detection results were compared with those of gas chromatography-mass spectrometry(GC-MS).The results showed that the qualitative detection of turmeric volatile oil could be completed by GC-SAW in 20 seconds,while GC-MS needed 30 minutes.The detection speed of GC-SAW was faster.At the same time,10 common chemical components were detected by GCSAW and GC-MS,which accounted for more than 90%of the total components.There were relatively a few chemical components detected by GC-SAW due to the short chromatographic column.In the quantitative analysis,the sample injection mode of GC-SAW was investigated.There was a significant difference between headspace injection and liquid injection in the detection of compound content.The quantitative analysis of trans-caryophyllene in turmeric volatile oil was carried out adopting liquid injection method consistent with GC-MS.The detection limit of trans-caryophyllene in turmeric volatile oil was 16.9 pg,and the ideal linear relationship was obtained with R=0:9976 in the range of 0.001624-0.01624 mg/mL,with excellent precision and repeatability.GC-SAW can be used for the rapid qualitative and quantitative analysis of turmeric volatile oil.It provides a novel and rapid detection technology basis for the analysis of volatile oil in traditional Chinese medicine.

关 键 词：姜黄挥发油 声表面波气相色谱仪 气相色谱-质谱仪 快速检测 定性分析 

分 类 号：O656.31[理学—分析化学]