梅毒血清固定患者外周血Th1,Th2,Treg和Th17相关细胞因子检测  

Analysis of Th1 cells, Th2 cells, Treg cells and Th17 cells related cytokines in peripheral blood of the serofast syphilis patients

作　　者：温晶[1] 李珍[1] 赵伟[1] 李富容[1] 侯建玲[1] 李中伟[1] 郑荣涛[1] 田洪青[1] WEN Jing;LI Zhen;ZHAO Wei;LI Furong;HOU Jianling;LI Zhongwei;ZHENH Rongtao;TIAN Hongqing(Shandong Provincial Hospital for Skin Diseases&Shandong Provincial Institute of Dermatology and Venereology,Shandong First Medical University,Jinan 250022,China)

机构地区：[1]山东第一医科大学附属皮肤病医院(山东省皮肤病医院,山东省皮肤病性病防治研究所),山东济南250022

出　　处：《中国麻风皮肤病杂志》2021年第6期356-359,共4页China Journal of Leprosy and Skin Diseases

基　　金：山东省重点研发计划(编号:2018CXGC1203);山东省医药卫生科技发展计划项目(编号:2016WS0453)。

摘　　要：目的:探讨梅毒血清固定患者Th1,Th2,Treg和Th17相关细胞因子与梅毒血清固定的关系。方法:采用酶联免疫吸附试验法(ELISA)分别对不同组(梅毒血清固定组、新发梅毒组、梅毒治疗后转阴组和正常对照组)人群外周血进行Th1、Th2、Treg和Th17相关细胞因子(IFN-γ、IL-2、IL-12、IL-4、IL-10、IL-6、IL-17、IL-22、TGF-β的)检测,并进行相关性分析。结果:与新发梅毒组、梅毒治疗后转阴组以及对照组比较,梅毒血清固定组IFN-γ、IL-2和IL-12水平均降低,差异均有统计学意义(均P<0.05);但IL-4、IL-10及TGF-β水平均升高,差异均有统计学意义(均P<0.05)。血清固定组IL-17水平低于新发梅毒组和转阴组(均P<0.05);IL-22低于新发梅毒组(P<0.05);IL-6高于对照组(P<0.05)。结论:梅毒血清固定患者可能同时存在Th1细胞/Th2细胞,Treg细胞/Th17细胞的失衡,从而影响患者体内整体免疫应答水平。Objective:To investigate the relationship between Th1,Th2,Treg and Th17 related cytokines and serofast syphilitic.Methods:The levels of Th1,Th2,Treg and Th17-related cytokines(IFN-γ,IL-2,IL-12,IL-4,IL-10,IL-6,IL-17,IL-22,TGF-β)in peripheral blood of different groups(serofast syphilis,syphilis,syphilis seroconvert and control)were detected by ElISA.Results:The levels of IFN-γ,IL-2 and IL-12 were lower and the levels of IL-4,IL-10 and TGF-βwere higher in the Syphilis serofast group than in the syphilis group,syphilis seroconvert group and control group,with significant differences(P s<0.05).The level of IL-17 in serofast syphilis group was significantly lower than that in syphilis group and control group(P s<0.05),while IL-22 in serofast syphilis group was significantly lower than that in syphilis group(P<0.05),IL-6 in syphilis serofast group was significantly higher than that in control group(P<0.05).Conclusion:The imbalance of Th1,Th2,Treg and Th17 cells and TH17 cells may exist in the patients with serofast syphilis,which affects the whole immune response level of the patients.

关 键 词：梅毒血清固定 TH1细胞 TH2细胞 TREG细胞 Th17细胞 细胞因子 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]