非洲猪瘟血清学诊断靶点的研究进展  

Advances Research on African Swine Fever Serological Diagnostic Targets

作　　者：杨文兵 邹亚文[1] 蒋一凡 余婉婷 杨毅[1] 邬静[1] 王乃东[1] YANG Wenbing;ZOU Yawen;JIANG Yifan;YU Wanting;YANG Yi;WU Jing;WANG Naidong(College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China;Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines,Changsha 410128,China;R&D Center for Animal Reverse Vaccinology of Hunan Province,Changsha 410128,China)

机构地区：[1]湖南农业大学动物医学院,长沙410128 [2]兽用蛋白质工程疫苗湖南省重点实验室,长沙410128 [3]兽用疫苗逆向创制湖南省工程研究中心,长沙410128

出　　处：《畜牧兽医学报》2021年第5期1208-1217,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：湖南省重点领域研发计划(2019 NK2171)。

摘　　要：非洲猪瘟(African swine fever,ASF)是一种由非洲猪瘟病毒(African swine fever virus,ASFV)引起的猪高致死性传染病。ASFV编码蛋白p30、p54和p72等具有较高的免疫原性,且部分氨基酸序列较为保守,常被作为血清学诊断靶点,用于评价ASF不同阶段或发病程度的抗体水平变化,ASF血清学诊断靶点相关深入研究有助于其感染检测、致病机制和机体免疫系统反应等探究。本文系统阐述用于ASF血清学诊断的主要候选蛋白及其靶点的检测应用进展,并分析候选靶点的抗原表位(区域)及其抗体结合的特点,探讨其作为ASF血清学诊断新靶点的应用潜力。African swine fever(ASF)is a highly fatal infectious disease caused by African swine fever virus(ASFV)in pigs.The proteins p30,p54 and p72 encoded by ASFV have high immunogenicity and some amino acid sequences are conserved,so they are often used as serological diagnostic targets to evaluate the changes of antibody levels through different stages or degrees of ASFV infection.In-depth study of ASF serological diagnostic targets would be helpful to explore its infection detection,pathogenic mechanism and immune system response.This paper systematically reviewed the progress in the detection of main candidate proteins and their targets for serological diagnosis of ASF,analyzed the antigenic epitopes(regions)of candidate targets and the characteristics of antibody binding,and discussed their application potential as new targets for serological diagnosis of ASF.

关 键 词：非洲猪瘟病毒(ASFV) 诊断靶点 结构蛋白 抗原表位 

分 类 号：S852.659.1[农业科学—基础兽医学]