26例犬毛囊肿瘤的组织病理学诊断和免疫组织化学分析  

Histopathological Diagnosis and Immunohistochemical Analysis of 26 Cases of Canine Hair Follicle Tumors

作　　者：陈梦月 张羽晨 娄江城 罗传真 赵红利 刘晓丽[1] 胡薛英[1] CHEN Mengyue;ZHANG Yuchen;LOU Jiangcheng;LUO Chuanzhen;ZHAO Hongli;LIU Xiaoli;HU Xueying(College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学动物医学院,武汉430070

出　　处：《畜牧兽医学报》2021年第5期1407-1413,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：本研究旨在为犬毛囊肿瘤的诊断提供最优的免疫标记物,提高肿瘤诊断的准确性,缩短肿瘤的病理学诊断时间,为犬毛囊肿瘤病的临床精准治疗提供帮助。作者收集了26例临床犬毛囊肿瘤病例,分别用免疫标记物CK5/6、CK8/18、CK19、P63、CD34、CD10、Vimentin对肿瘤样本进行免疫组织化学标记(IHC)。26例犬毛囊肿瘤的IHC染色结果表明,CD34在86.9%的毛囊肿瘤中呈瘤细胞阳性;CD10和CK8/18在毛上皮瘤中呈瘤细胞阳性,在其他毛囊肿瘤中均为瘤细胞阴性;CK19在良性和恶性毛上皮瘤中分别为强阳性和中阳性,CD34在良性和恶性毛上皮瘤中分别为中阳性和弱阳性;CD10在毛母细胞瘤中呈周边间质特异性阳性;Vimentin在毛囊肿瘤中呈瘤细胞阴性,间质细胞阳性。结果显示,CK5/6、P63可用作本研究中所有毛囊肿瘤的阳性标记物;CD34可用作除毛母质瘤和毛囊瘤外的其他毛囊肿瘤的瘤细胞特异性阳性标记物;CD10和CK8/18可用作毛上皮瘤的瘤细胞阳性标记物;共同使用CK19和CD34可用于区分毛上皮瘤的良恶性;CD10可用作毛母细胞瘤周边间质特异性阳性标记物;Vimentin可用作毛囊肿瘤的瘤细胞阴性标记物。This study aims to provide the best immune markers for the diagnosis of canine hair follicle tumors, improve the accuracy and shorten the time of tumor diagnosis, and be helpful for the clinical precise treatment. In this study, 26 cases of clinical canine hair follicle tumors were collected, and the tumor cases were labeled with immunohistochemistry(IHC) using immune markers CK5/6, CK8/18, CK19, P63, CD34, CD10, and Vimentin. The results of IHC staining of 26 tumors showed that CD34 was positive in 86.9% follicular tumors;Tumor cells of trichoepitheliomas were specifically positive for CD10 and CK8/18;CK19 was strongly positive in benign trichoepitheliomas and moderately positive in malignant tumors, and CD34 was moderately positive in benign trichoepitheliomas and weakly positive in malignant tumors;CD10 was specifically positive in the peripheral stroma of piloblastoma;Tumor cells in all cases were negative for Vimentin, while mesenchymal cells were positive. The results showed that CK5/6 and P63 can be used as positive markers of all follicular tumors in this study. CD34 can be used as a specific positive marker of hair follicular tumors except pilomatricomas and trichofolliculomas. CD10 and CK8/18 can be used as positive markers of trichoepitheliomas. CK19 and CD34 can be used to distinguish the benign and malignant trichoepitheliomas. CD10 can be used as a specific positive marker of the surrounding stroma of trichoblastomas. Vimentin can be used as a negative marker of hair follicle tumors.

关 键 词：犬 毛囊肿瘤 组织病理学 免疫组织化学 诊断 免疫标志物 

分 类 号：S857.4[农业科学—临床兽医学]