联合检测尿总蛋白避免尿微量白蛋白钩状效应的方法  

Combined detection of urinary total protein to avoid the hook effect of urinary microalbumin

作　　者：旦曲[1] 罗桑次珍 泽仁曲措 马超超 刘治娟[1] Danqu;Luosangcizhen;Zerenqucuo;MA Chaochao;LIU Zhijuan(Department of Clinical Laboratory,Tibet Autonomous Region People's Hospital,Lhasa 850000,Tibet,China;Department of Clinical Laboratory,Peking Union Medical College Hospital,Beijing 100730,China)

机构地区：[1]西藏自治区人民医院检验科,西藏拉萨850000 [2]北京协和医院检验科,北京100730

出　　处：《检验医学》2021年第5期477-479,共3页Laboratory Medicine

基　　金：西藏自治区自然科学基金组团式援藏项目(XZ2017ZR-ZYZ13)。

摘　　要：目的分析尿总蛋白(TP)和尿微量白蛋白(mAlb)的相关性,建立避免钩状效应导致的尿mAlb假阴性的方法。方法收集1711例患者尿mAlb和尿TP检测数据,采用Spearman相关分析评估尿mAlb与尿TP的相关性,建立线性回归方程。采用35份检测尿mAlb时有钩状效应的尿液样本进行验证。结果尿TP与尿mAlb呈正相关(r=0.880,P=0.000)。以尿TP为因变量(Y),尿mAlb为自变量(X),建立的线性回归方程为:Y=116.361+1.245X(r^(2)=0.921,P=0.000)。将发生钩状效应的35份样本的尿mAlb检测值(记为尿mAlb钩状)代入方程,计算得到尿TP值(记为尿TP_(钩状)),随后将尿液样本稀释并检测尿mAlb,将检测结果记为尿mAlb稀释,代入方程后计算尿TP值(记为尿TP_(稀释))。尿TP_(实测)与尿TP_(钩状)差异有统计学意义(P<0.001),与尿TP_(稀释)差异无统计学意义(P>0.05)。结论建立尿TP与尿mAlb的线性回归方程可简单地判断尿mAlb检测时是否存在钩状效应。该方法简单、不受仪器和试剂的限制,可在临床上推广。Objective To analyze the correlation between urinary total protein(TP)and urinary microalbumin(mAlb),and to find a method for avoiding the false negativity of urinary mAlb caused by hook effect.Methods The results of urinary mAlb and urinary TP of 1171 patients were collected,and the correlation between them was evaluated by Spearman correlation analysis.Linear regression equation was established.Totally,35 urine samples with hook effect were validated for urinary mAlb.Results Urinary TP was positively correlated with urinary mAlb(r=0.880,P=0.000).Taking urinary TP as dependent variable(Y),urinary mAlb as independent variable(X),the linear regression equation was established,Y=116.361+1.245X(r^(2)=0.921,P=0.000).By substituting the urinary mAlb determination values of 35 samples with hook effect(recorded as urinary mAlbhook)into the equation,urinary TP(recorded as urinary TP_(hook))was calculated,urine samples were diluted,and urinary mAlb was determined.The results were recorded as urinary mAlbdilution.After substituting the equation,the urinary TP_(value)(recorded as urinary TP_(dilution))was calculated.There was statistical significance between urinary TP_(measured) and urinary T_(Phook)(P<0.001),and there was no statistical significance with urinary TPdilution(P>0.05).Conclusions According to establishing the linear regression equation between urinary TP and urinary mAlb,whether there is a hook effect in urinary mAlb deterimination can be determined simply.This method is simple and not limited by instruments and reagents,which can be popularized in clinic.

关 键 词：微量白蛋白 总蛋白 尿液 钩状效应 

分 类 号：R446.1[医药卫生—诊断学]