基于氮掺杂碳量子点荧光探针检测四环素  

Fluorescence detection of tetracycline based on nitrogen doped carbon quantum dots

作　　者：李义梅 陈谦 LI Yi-mei;CHEN Qian(Yan'an Institute for drug control,Yan'an 716000,China;College of Chemistry and Chemical Engineering,Yan'an University,Yan'an 716000,China)

机构地区：[1]延安市药品检验所,陕西延安716000 [2]延安大学化学与化工学院,陕西延安716000

出　　处：《化学研究与应用》2021年第5期813-818,共6页Chemical Research and Application

基　　金：陕西省延安市科技发展基金项目(YDZ2013-11)资助;陕西省教育厅重点实验室项目(20JS156)资助。

摘　　要：本文以淀粉为碳源,以L-酪氨酸为氮供体,通过一步水热法制备了氮掺杂碳量子点(N-CQDs)。研究表明,N-CQDs表面富含氨基、羧基、羟基等官能团,说明具有很好的水溶性。还发现在pH=6.00的KH2PO4-NaOH缓冲溶液中,四环素对N-CQDs有强荧光猝灭作用,且四环素浓度在1.6~16μmol·L^(-1)范围和16~100μmol·L^(-1)范围内与N-CQDs的荧光猝灭的强度呈现较好的线性关系,检出限为0.45μmol·L^(-1),依此构建了N-CQDs荧光探针测定四环素的方法。该方法用于四环素片含量的测定,回收率为98.4%~102%。Starch carbon quantum dots(N-CQDs)doped with L-Tyrosine nitrogen were prepared by one-step hydrothermal method.The results showed that N-CQDs were rich in functional groups such as amino,carboxyl and hydroxyl groups,indicating that N-CQDs had good water solubility.It was also found that tetracycline had strong fluorescence quenching effect on N-CQDs in the pH=6.00 KH2PO4-NaOH buffer solution,and the concentration of tetracycline in the range of 1.6~16μmol·L^(-1)and 16~100μmol·L^(-1) showed a good linear relationship with the fluorescence quenching intensity of N-CQDs,and the detection limit was 0.45μmol·L^(-1).The method was applied to the determination of tetracycline,and the recovery was 98.4%~102%.

关 键 词：氮掺杂碳量子点 水热法 荧光猝灭 四环素 

分 类 号：O657.3[理学—分析化学]