瓜子金皂苷己通过SOCS3抑制氧糖剥夺/复氧所致小胶质细胞的炎症反应  

The inflammatory response of microglia induced by oxygen-glucose deprivation/reoxygenation and inhibited through SOCS3 by Polygalasaponin F

作　　者：高燕 付旭阳 贺帅[3] 高冰[2] 石瑞丽[1,2,4] GAO Yan;FU Xuyang;HE Shuai;GAO Bing;SHI Ruili(Department of Physiology,Baotou Medical College,Inner Mongolia,Baotou 014040,China;Institute of Neuroscience,Baotou Medical College;Department of Pathology,Baotou Medical College;Key Laboratory of Hypoxic Translational Medicine of Inner Mongolia;The People’s Hospital of Dalate,Ordos)

机构地区：[1]包头医学院基础医学与法医学院生理学教研室,内蒙古包头014040 [2]包头医学院神经科学研究所 [3]包头医学院基础医学与法医学院病理学教研室 [4]内蒙古自治区低氧转化医学重点实验室 [5]达拉特旗人民医院检验科

出　　处：《包头医学院学报》2021年第4期64-68,共5页Journal of Baotou Medical College

基　　金：2020年度内蒙古人才开发基金项目;国家自然科学基金项目(81960734);内蒙古自然科学基金项目(2017MS0808)。

摘　　要：目的:观察瓜子金皂苷己(Polygalasaponin F,PGSF)是否影响氧糖剥夺/复氧(Oxygen and glucose deprivation/reoxygenation,OGD/R)处理的BV-2小胶质细胞炎性细胞因子及细胞因子信号抑制因子3(Suppressors of cytokine signaling3,SOCS3)表达,初步探讨PGSF对抗OGD/R炎症反应的机制。方法:构建BV-2小胶质细胞OGD/R体外模型,分为正常对照组、模型组、PGSF低剂量组、PGSF中剂量组及PGSF高剂量组。BV-2细胞氧糖剥夺2 h、复糖复氧6 h后提取总RNA,通过实时荧光定量多聚酶链式反应检测BV-2细胞炎性因子TNF-α、IL-1β、IL-6以及SOCS3的基因表达。结果:I/R后BV-2细胞中炎性因子TNF-α、IL-1β、IL-6及SOCS3的mRNA表达明显增加;给予PGSF处理可以逆转上述观察指标的改变。结论:PGSF在体外实验中具有抑制神经炎症的作用,SOCS3信号通路可能参与介导了PGSF的抗炎作用。Objective:To investigate the effects of polygalasaponin F(PGSF)on the expression of inflammatory cytokines and suppressors of cytokine signaling 3(SOCS3)in BV-2 microglia treated with oxygen and glucose deprivation/reoxygenation(OGD/R),and to preliminarily explore the mechanism of PGSF against OGD/R inflammation.Methods:OGD/R models of BV-2 microglia were established in vitro and divided into the normal control group,the model group,the low-dose PGSF group,the medium-dose PGSF group and the high-dose PGSF group.Total RNA was extracted from BV-2 cells after oxygen glucose deprivation for 2 h and reoxygenation for 6 h,with the gene expressions of TNF-α,IL-1β,IL-6 and SOCS3 in BV-2 cells detected by real-time fluorescent quantitative polymerase chain reaction(qPCR).Results:The mRNA expressions of TNF-α,IL-1β,IL-6 and SOCS3 in BV-2 cells were significantly increased after OGD/R insult and PGSF treatment could reverse these changes.Conclusion:PGSF can inhibit neuroinflammation through in vitro experiments,and the SOCS3 signaling pathway may be involved in the anti-inflammatory effect of PGSF.

关 键 词：氧糖剥夺/复氧 瓜子金皂苷己 BV-2细胞 炎症 细胞因子信号抑制因子3 

分 类 号：R285.5[医药卫生—中药学]