重组酶聚合酶扩增技术在植物病原快速检测中的应用  

Recombinase polymerase amplification and its applications in quick detection of plant pathogens

作　　者：马文娣 田逸英 焦志远 周涛[1] 范在丰[1] MA Wendi;TIAN Yiying;JIAO Zhiyuan;ZHOU Tao;FAN Zaifeng(Department of Plant Pathology, China Agricultural University, Beijing 100193, China)

机构地区：[1]中国农业大学植物病理学系,北京100193

出　　处：《植物保护》2021年第3期1-5,13,共6页Plant Protection

基　　金：国家重点研发计划(2016YFD0300710)。

摘　　要：植物病原的快速检测对于植物病害防控具有重要意义。重组酶聚合酶扩增(recombinase polymerase amplification,RPA)是近年建立的等温核酸扩增技术,具有灵敏度高、特异性强、适用于现场快速检测等特点,已在多个领域广泛应用。本文对重组酶聚合酶扩增技术的原理、特性、检测方法及其在植物病原体检测领域的应用进展加以综述。The rapid diagnosis of plant pathogens is essential for efficient prevention and control of plant diseases.Recombinase polymerase amplification(RPA)is an isothermal nucleic acid amplification technology developed in recent years,which has been widely applied in many fields.It is suitable for on-site rapid detection with high sensitivity and strong specificity.In this paper,we review the principles,characteristics,detection methods of RPA and its applications in the detection of plant pathogens.

关 键 词：重组酶聚合酶扩增 植物病原 检测 

分 类 号：S474[农业科学—农业昆虫与害虫防治]