黏虫MagR和Cry2基因的时空表达分析  

Temporal and spatial expression of MagR and Cry2 genes in Mythimna separata

作　　者：谢殿杰 张蕾[1] 程云霞[1] 江幸福[1] XIE Dianjie;ZHANG Lei;CHENG Yunxia;JIANG Xingfu(State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《植物保护》2021年第3期6-13,共8页Plant Protection

基　　金：国家自然科学基金(31672019,31871951);国家绿肥产业技术体系(CARS-22);国家重点研发计划(2017YFD0201701,2017YFD0201802);北京市自然科学基金(6172030)。

摘　　要：为了明确黏虫MagR、Cry2基因在定向行为中的作用,本研究采用基因序列分析及实时荧光定量技术,研究了黏虫MagR、Cry2基因的基因序列特征及其表达模式。结果表明,黏虫MagR基因编码131个氨基酸,蛋白分子量为14.17 kD,等电点为9.3,具有多个铁硫簇结合位点;黏虫Cry2基因编码757个氨基酸,蛋白分子量为87.04 kD,等电点为8.73。羽化后不同日龄迁飞型黏虫成虫各部位Cry2基因表达量存在显著差异。羽化后3 d成虫头部和胸部Cry2基因表达量显著高于其他日龄。迁飞型黏虫成虫羽化后不同日龄各部位MagR的表达量具有显著性差异;5 d时各部位的表达量达到最高,各日龄头部和胸部的表达量均显著高于腹部。In order to clarify the role of MagR and Cry2 genes in the orientation behavior of Mythimna separata,the gene sequence analysis and qRT-PCR were performed to study the gene sequence characteristics and expression patterns of MagR and Cry2 genes.The results showed that MagR gene encoded 131 amino acids,with a molecular weight of 14.17 kD,an isoelectric point of 9.3 and many iron sulfur cluster binding sites.The Cry2 gene encoded 757 amino acids,with a molecular weight of 87.04 kD and an isoelectric point of 8.73.After eclosion,there were significant differences of Cry2 gene expression in different parts of M.separata at different day-ages.The Cry2 gene expression in the head and thorax of 3-day-old adults was significantly higher than that in the adults of other day-ages.Meanwhile,after eclosion,there were also significant differences of MagR expression in different parts of M.separata at different day-ages.At 5-day-age,the expression level of each part reached the highest,and the expression levels in the head and thorax at each age were significantly higher than those in the abdomen.

关 键 词：黏虫 时空表达 定向 CRY2 MagR 

分 类 号：S433[农业科学—农业昆虫与害虫防治]