近零磁场下东方黏虫转录组分析  

Transcriptome analysis of the oriental armyworm Mythimna separata in a near-zero magnetic field

作　　者：闫蒙蒙 张蕾[1] 程云霞[1] 江幸福[1] YAN Mengmeng;ZHANG Lei;CHENG Yunxia;JIANG Xingfu(State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《植物保护》2021年第3期37-43,53,共8页Plant Protection

基　　金：国家自然科学基金(31672019,31871951);国家绿肥产业技术体系(CARS-22);国家重点研发计划(2017YFD0201701,2017YFD0201802);北京市自然科学基金(6172030)。

摘　　要：为进一步探究黏虫的磁感应机制,明确磁场强度变化对黏虫相关基因表达的影响及相应的分子机制,本研究利用亥姆霍兹线圈装置产生近零磁场,使用Illumina技术,分别对近零磁场(小于500 nT)和地磁场(约54000 nT)下饲养的黏虫雌蛾和雄蛾进行转录组测序分析。结果表明:相对于地磁场,近零磁场下雌、雄蛾分别鉴定出1670和1401个差异表达基因,其中雌蛾鉴定出614个上调基因,1056个下调基因;雄蛾鉴定出545个上调基因,856个下调基因;雌、雄蛾共同差异表达基因274个。GO功能分析和pathway结果显示近零磁场胁迫与离子结合、催化和代谢等关键过程密切相关。对选取的10个差异基因进行荧光定量PCR验证,证明了转录组测序分析结果准确可靠。本试验为深入探究迁飞性昆虫的地磁定向机制提供了理论基础。In order to further explore the mechanism of magnetoreception of Mythimna separata and clarify the effects of magnetic field changes on the expression of related genes,transcriptome analysis of M.separata in a near-zero magnetic field was carried out.In this study,Helmhortz coils were used to generate a near-zero magnetic field,and the Illumina technology was used to sequence the transcriptome of M.separata moths(female and male)raised in near-zero magnetic field(less than 500 nT)and normal geomagnetic field(about 54000 nT).The results showed that compared with geomagnetic field,1670 and 1401 differentially expressed genes were identified in female and male adults under near-zero magnetic field,respectively,among which 614 up-regulated genes and 1056 down-regulated genes were identified in female adults,and 545 up-regulated genes and 856 down-regulated genes were identified in male adults.There were 274 common differentially expressed genes in male and female adults.The gene ontology(GO)functional annotation and pathway enrichment analysis results showed that near-zero magnetic field was related to key biological processes,such as ion binding,catalysis activity and metabolism process.The results of qRT-PCR showed that the transcriptome was accurate and reliable.Our study provided a theoretical fundament for further study of the mechanism of magnetoreception in migratory insects.

关 键 词：近零磁场 地磁场 黏虫 转录组 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]