核桃JrGRAS基因家族鉴定与响应低温胁迫基因筛选  

Identification of walnut JrGRAS gene family and screening of genes in response to low temperature stress

作　　者：刘凯 赵星哲 王红霞 赵书岗[3] 张俊佩[4] 马庆国[4] 张志华 LIU Kai;ZHAO Xingzhe;WANG Hongxia;ZHAO Shugang;ZHANG Junpei;MAQingguo;ZHANG Zhihua(College of Horticulture,Hebei Agricultural University,Baoding 071001,China;Hebei Agricultural Technology Innovation Center,Baoding 071001,China;College of Life Sciences,Hebei Agricultural University,Baoding 071001,China;Research Institute of Forestry,Chinese Academy of Forestry/Key Laboratory of Tree Breeding and Cultivation of the State Forestry and Grassland Administration,Beijing 100091,China)

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]河北省山区农业技术创新中心,河北保定071001 [3]河北农业大学生命科学学院,河北保定071001 [4]中国林业科学研究院林业研究所/国家林业和草原局林木培育重点实验室,北京100091

出　　处：《河北农业大学学报》2021年第2期27-40,共14页Journal of Hebei Agricultural University

基　　金：国家重点研发计划项目(2019YFD1001603);国家自然基金(32072524,31672116);河北省自然科学基金(C2019204270)。

摘　　要：为深入解析核桃GRAS基因家族在低温胁迫中的生物学功能,本研究根据生物信息学模型搜索核桃基因组中GRAS基因,采用ExPASy、MEME、GSDS、MEGA6.0、String和Plantcare等软件分析该家族成员理化性质、基因结构、保守基序、进化关系、启动子区域结合位点及蛋白功能互作等,分析GRAS基因家族在低温胁迫下表达模式。聚类分析发现,据拟南芥分类将核桃GRAS基因家族分为9个亚族,分别为HAM、DELLA、SCR、SCL、LISCL、LS、SHR、PAT1和NEWCLASS;每个成员蛋白磷酸化和糖基化的位点和数目各异,均存在丰富的激素信号和胁迫应答调控元件;蛋白互作预测发现JrGRAS和其他响应非生物胁迫蛋白可以发生互作。在低温胁迫48 h后经转录表达分析,JrGRAS8、JrGRAS14、JrGRAS15、JrGRAS20、JrGRAS33、JrGRAS39、JrGRAS41、JrGRAS48、JrGRAS49、JrGRAS51、JrGRAS59、JrGRAS63为差异表达基因,推测可能响应低温胁迫。本研究结果将为深入研究核桃GRAS基因功能奠定基础。In order to analyze the biological functions of walnut GRAS gene family in low temperature stress, this study searched for GRAS genes in walnut genome based on bioinformatics models by using software such as ExPASy, MEME, GSDS, MEGA6.0, String and Plantcare. Physical and chemical properties, gene structure, conservative motifs, evolutionary relationships, promoter region binding sites and protein functional interactions were analyzed to study the expression patterns of GRAS gene family under low temperature stress. Cluster analysis found that according to Arabidopsis classification, the walnut GRAS gene family was divided into 9 sub-families, namely HAM, DELLA, SCR, SCL, LISCL, LS, SHR, PAT1 and NEW CLASS;each member had different protein phosphorylation and glycosylation sites and numbers, and there were abundant hormone signals and stress response regulatory elements. Protein interaction prediction found JrGRAS and others proteins can interact in response to abiotic stress. After 48 h of low temperature stress, the transcriptional expression analysis showed that JrGRAS8, JrGRAS14, JrGRAS15, JrGRAS20, JrGRAS33, JrGRAS39, JrGRAS41, JrGRAS48, JrGRAS49, JrGRAS51, JrGRAS59, JrGRAS63 were differentially expressed genes, which were speculated to respond to low temperature stress. The results of this study will lay the foundation for in-depth study of walnut GRAS gene function.

关 键 词：核桃 GRAS 非生物胁迫 转录表达 

分 类 号：S664.1[农业科学—果树学]