高盐高油对餐厨垃圾厌氧发酵酶活性及产VFAs的影响  

Effects of high salt and high oil content on anaerobic fermentation enzyme activity and production of VFAs in food waste

作　　者：谷士艳[1] 闫屹嵩 张文一 孙继阳 张敏[1] 李轶[1] Gu Shiyan;Yan Yisong;Zhang Wenyi;Sun Jiyang;Zhang Min;Li Yi(Shenyang Agricultural University,School of Engineering,Shenyang 110866,China)

机构地区：[1]沈阳农业大学工程学院,沈阳110866

出　　处：《农业工程学报》2021年第6期228-234,共7页Transactions of the Chinese Society of Agricultural Engineering

基　　金：辽宁省自然基金面上项目(20170540813,2015020635);辽宁省教育厅项目;沈阳市科技局课题(18-013-0-86)。

摘　　要：为探究高盐高油对厌氧发酵产酸及相关酶活性的影响,该研究以餐厨垃圾为发酵原料,在接种物质量为30%、TS(Total Solids)为8%、温度为35℃、初始pH值为7的条件下进行批式厌氧发酵试验,研究盐、油质量浓度为5、7、9、11、13、16 g/L的6个条件对发酵过程中的SCOD(Solluted Chemical Oxigen Demand)、VFAs(Volatile Fatty Acids)、淀粉酶、蛋白酶、辅酶F420和脱氢酶活性的影响。结果表明:随着盐油浓度的提高,SCOD峰值下降了23%~38%,并出现了2~3 d的延迟;高盐高油条件下产酸以丁酸为主,丙酸、乙酸和异戊酸含量次之,正己酸、异丁酸、正戊酸的含量最低,不同盐油条件下VFAs各组分比例差距较小;相关酶活性峰值均降低了5%~35%,相关酶活性峰值均推迟了3~6 d出现,盐油浓度越高抑制现象越明显。研究结果可为高盐高油对厌氧发酵的影响和后续试验提供参考。Food waste is an excellent raw material for anaerobic fermentation,due to its rich in starch,protein,and high content of organic matter.But there are relatively high concentrations of salt and oil difficult to remove,particularly on a high removal cost in anaerobic fermentation.This study aims to explore the effects of high salt and oil in eat hutch garbage on the acid production and related enzyme activities of anaerobic fermentation.Taking food waste as the raw material in fermentation,a batch anaerobic fermentation test was carried out under the conditions of inoculation content of 30%,the Total Solid(TS)of 8%,the temperature of 35℃,and the initial pH of 7.A minimal inhibitory concentration test was selected to determine the high concentration in salt and oil.Initial raw materials were divided into six test groups for anaerobic fermentation according to the maximum concentration and gradient of salt and oil,including 5,7,9,11,13,16 g/L.A detection was performed on the Soluble Chemical Oxygen Demand(SCOD)in biogas slurry,to determine the type and content of Volatile Fatty Acids(VFAs),as well as activity change of amylase,protease,coenzyme F420,and dehydrogenase.The experimental results are as follows.The SCOD did not change,but the peak SCOD decreased by 23%-38%with a 2-3 d delay,indicating a more intense inhibition phenomenon with the increase of salt/oil concentration.The high salt/oil content delayed the occurrence time for the peak of VFAs,leading to the accumulation of VFAs.The accumulation peak of VFAs decreased first and then increased with the increase of the salt/oil content,where the accumulation concentration of VFAs decreased by 34.76%.Moreover,the decomposition of oil-produced VFAs contributed to the increase in the concentration peak of VFAs.Butyric acid(accounting for 54%-75%)was the main acid product under the condition of high salt/oil.There were similar contents(about 10%-20%)of propionic acid,acetic acid,and isovaleric acid.The lowest contents were only 1%-8%of n-hexanoic acid,isobutyric ac

关 键 词：垃圾 发酵 酶活性 盐分 油脂 挥发性脂肪酸(VFAs) 

分 类 号：X713[环境科学与工程—环境工程]