红景天苷对哮喘模型小鼠气道炎症的抑制作用及其机制  

Effect of salidroside on airway inflammation in mice with asthma and its mechanism

作　　者：栾雪 延光海 李海波[1] 张波[1] 张巍[3] 黄园园[1] LUAN Xue;YAN Guanghai;LI Haibo;ZHANG Bo;ZHANG Wei;HUANG Yuanyuan(Department of Pediatrics,First Hospital,Jilin University,Changchun 130021,China;Key Laboratory of Immunization and Targeting of Common Allergic Diseases of Jilin Province,Yanji 133002,China;Department of Child Health Management Center,Changchun Children's Hospital,Changchun 130051,China)

机构地区：[1]吉林大学第一医院儿科,吉林长春130021 [2]吉林省过敏性常见疾病免疫与靶向重点实验室,吉林延吉133002 [3]吉林省长春市儿童医院健康管理中心,吉林长春130051

出　　处：《吉林大学学报（医学版）》2021年第3期537-544,共8页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金项目(81860729)。

摘　　要：目的:探讨红景天苷(SAL)对小鼠急性哮喘气道炎症的干预作用,并阐明其作用机制。方法:将50只清洁级BALB/c雌性小鼠随机分为对照组、卵清蛋白(OVA)组、低剂量SAL组、高剂量SAL组和地塞米松组,每组10只。于实验第1、7和14天,腹腔注射混合液200μL(由生理盐水、10 mg OVA和1 mg氢氧化铝组成)建立小鼠哮喘模型,第21天时,将致敏小鼠置于实验玻璃箱内,以0.1 g OVA+10 mL生理盐水雾化形式激发30 min,每日1次,共7 d。低和高剂量SAL组小鼠在每次激发免疫前1 h分别腹腔注射30和60 mg·kg^(-1)SAL治疗液,地塞米松组小鼠腹腔注射2 mg·kg^(-1)地塞米松治疗液,而对照组小鼠以同剂量生理盐水代替。检测各组小鼠增强呼吸间歇(Penh)值并评估小鼠气道反应性,HE染色法观察各组小鼠肺组织形态表现,直接计数法计算各组小鼠肺泡灌洗液(BALF)中中性粒细胞、嗜酸性粒细胞和淋巴细胞数,ELISA法检测各组小鼠BALF中白细胞介素1β(IL-1β)、白细胞介素4(IL-4)、白细胞介素13(IL-13)、白细胞介素17A(IL-17A)、白细胞介素18(IL-18)和白细胞介素33(IL-33)水平,Western blotting法测定各组小鼠肺组织中NOD样受体家族蛋白3(NLPR3)、凋亡相关微粒蛋白(ASC)、Caspase-1、白细胞介素1β前体(pro-IL-1β)和IL-1β蛋白表达水平。结果:与对照组比较,OVA组小鼠Penh值明显升高(P<0.05);与OVA组比较,高剂量SAL组小鼠Penh值升高(P<0.05)。与对照组比较,OVA组小鼠气道平滑肌增厚并可见大量炎性细胞浸润;与OVA组比较,高剂量SAL组小鼠气道周围炎性细胞数减少(P<0.05)。与对照组比较,OVA组小鼠BALF中中性粒细胞、嗜酸性粒细胞和淋巴细胞数增加(P<0.05),IL-1β、IL-4、IL-13、IL-17A、IL-18和IL-33表达水平明显升高(P<0.05);与OVA组比较,高剂量SAL组小鼠BALF中中性粒细胞、嗜酸性粒细胞以和淋巴细胞数减少(P<0.05),IL-1β、IL-4、IL-13、IL-17A、IL-18和IL-33水平降低(P<0.05)。与对Objective:To explore the interventional effect of salidroside(SAL)on acute asthmatic airway inflammation in the mice,and to clarity its mechanism.Methods:Fifty clean BALB/c female mice were randomly divided into control group,ovalbumin(OVA)group,low dose of SAL group,high dose of SAL group and dexamethasone group,and there were 10 mice in each group.The mouse asthma model was established by intraperitoneal injection of 200μL mixed solution(containing normal saline,10 mg OVA,and 1 mg aluminum hydroxide)on the 1st,7th and 14th days.On the 21st day,the sensitized mice were placed in the experimental glass box.In the box,the mice were stimulated with 0.1 g OVA+10 mL physiological saline pulverization for 30 min,once a day,lasted for 7 d.The mice in low and high doses of SAL groups were intraperitoneally injected with 30-60 mg·kg^(-1) SAL treatment solution 1 h before each challenge,and the mice in dexamethasone group were intraperitoneally injected with 2 mg·kg^(-1) dexamethasone treatment solution,while the mice in control group were replaced with the same dose of saline.The enhanced pause(Penh)values of the mice in various groups were detected and the airway reactivities of the mice were evaluated;the morphology of lung tissue of the mice in various groups was observed by HE staining;the numbers of neutrophils,eosinophils and lymphocytes in alveolar lavage fluid(BALF)of the mice in various groups were calculated by direct counting method;ELISA method was used to detect the levels of interleukin 1β(IL-1β),interleukin 4(IL-4),interleukin 13(IL-13),interleukin 17A(IL-17A),interleukin 18(IL-18),and interleukin 33(IL-33)in BALF of the mice in various groups;the expression level of NOD-like receptor family protein 3(NLPR3),apoptosis-associated speck-like protein containing a CARD(ASC),Caspase-1,interleukin-1βprecursor(pro-IL-1β)and IL-1βproteins in lung tissue of the mice in various groups were determined by Western blotting method.Results:Compared with control group,the Penh value of the mice in OVA group was in

关 键 词：红景天苷 哮喘 气道炎症 NOD样受体家族蛋白3 凋亡相关微粒蛋白 

分 类 号：R562.25[医药卫生—呼吸系统]