电针预处理对心肌缺血再灌注损伤大鼠下丘脑外侧区和小脑顶核多巴胺、5-羟色胺含量的影响  

Effect of electroacupuncture preconditioning on the contents of dopamine and 5-hydroxytryptamine in lateral hypothalamus area and cerebellar fastigial nucleus of rats with myocardial ischemia-reperfusion injury

作　　者：余情[1,2] 蔡荣林 邵雪芳 张娅婷 魏小桐 胡玲 YU Qing;CAI Rong-lin;SHAO Xue-fang;ZHANG Ya-ting;WEI Xiao-tong;HU Ling(College of Acupuncture and Moxibustion and Tuina,Anhui University of CM,Hefei 230012,China;Graduate School,Anhui University of CM,Hefei 230012,China;Research Institute of Acupuncture and Moxibustion,Anhui Academy of TCM,Hefci 230038;Anhui Key Laboratory ofAcupuncture-Moxibustion Basis and Technology,Hefei 230038)

机构地区：[1]安徽中医药大学针灸推拿学院,合肥230012 [2]安徽中医药大学研究生院,合肥230012 [3]安徽省中医药科学院针灸经络研究所,合肥230038 [4]针灸基础与技术安徽省重点实验室,合肥230038

出　　处：《中国针灸》2021年第5期525-530,共6页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金资助项目:81774414、81973757;安徽省高校自然科学研究重点项目:KJ2019A0455;安徽省高校优秀拔尖人才培育资助项目:gxgwfx2019025。

摘　　要：目的:观察电针心经腧穴预处理对急性心肌缺血再灌注损伤(MIRI)大鼠下丘脑外侧区(LHA)与小脑顶核(FN)多巴胺(DA)、5-羟色胺(5-HT)含量的影响,探讨LHA和FN在电针心经腧穴抗急性MIRI效应中的作用及机制。方法:将60只SD大鼠随机分为假手术组、模型组、电针心经组、电针肺经组,每组12只;LHA+心经组(损毁双侧LHA)、FN+心经组(损毁双侧FN),每组6只。核团损毁3 d后,电针心经组、LHA+心经组、FN+心经组电针"神门""通里",电针肺经组电针"太渊""列缺",刺激电压为1 V,频率为2 Hz,每次20 min,每日1次,模型复制前共刺激7 d。除外假手术组,其他各组均采用冠状动脉左前降支结扎法复制MIRI大鼠模型。应用Power lab多导生理记录仪记录造模前、结扎后30 min及再灌注120 min的ST段位移值,采用高效液相色谱-电化学检测分析系统测定各组大鼠造模后LHA、FN透析液中DA、5-HT含量。结果:结扎后30 min、再灌注120 min时各组ST段位移值比较,模型组高于假手术组(P<0.01),电针心经组、LHA+心经组、FN+心经组和电针肺经组低于模型组(P<0.01),电针心经组低于电针肺经组、LHA+心经组和FN+心经组(P<0.01)。模型组大鼠FN、LHA中DA、5-HT含量低于假手术组(P<0.01);除外损毁对应的核团组,各干预组FN、LHA中DA、5-HT含量均高于模型组(P<0.01);电针心经组大鼠FN中DA、5-HT含量高于电针肺经组、LHA+心经组(P<0.01),LHA中5-HT含量高于电针肺经组和FN+心经组、DA含量高于电针肺经组(P<0.01)。结论:电针心经腧穴预处理可以有效减轻急性MIRI大鼠的心肌损伤,LHA、FN中DA、5-HT可能是其重要的物质基础。Objective To observe the effect of electroacupuncture(EA)preconditioning at heart meridian acupoints on the contents of dopamine(DA)and 5-hydroxytryptamine(5-HT)in lateral hypothalamus area(LHA)and cerebellar fastigial nucleus(FN)in the rats with acute myocardial ischemia-reperfusion injury(MIRI),and explore the role and mechanism of LHA and FN in the effect of EA at heart meridian acupoints against acute MIRI.Methods Sixty SD rats were randomly divided into a sham-operation group,a model group,an EA heart meridian group and an EA lung meridian group,12 rats in each group,as well as an LHA plus heart meridian group(damage of bilateral LHA)and an FN plus heart meridian group(damage of bilateral FN),6 rats in each one.Three days after nucleus destruction,EA was applied to"Shenmen"(HT 7)and"Tongli"(HT 5)in the EA heart meridian group,the LHA plus heart meridian group and the FN plus heart meridian group and EA was applied to"Taiyuan"(LU 9)and"Lieque"(LU 7)in the EA lung meridian group,with 1 V in stimulating voltage and 2 Hz in frequency,lasting 20 minutes each time,once a day,for consecutively 7 days before model replication.Except in the sham-operation group,MIRI rat models were duplicated by ligation of the left anterior descending branch of the coronary artery in the rest groups.Using Power lab physiological recorder,ST segment displacement value was recorded before modeling,30 min after ligation and 120 min after reperfusion separately.The high performance liquid chromatography-electrochemical detection and analysis system was adopted to determine the contents of DA and 5-HT in LHA and FN dialysate after rat modeling in each group.Results In comparison of ST segment displacement value 30 min after ligation and 120 min after reperfusion among groups,the value in the model group was higher than that in the sham-operation group(P<0.01),those in the EA heart meridian group,the LHA plus heart meridian group,the FN plus heart meridian group and the EA lung meridian group were lower than those in the model group succe

关 键 词：心肌缺血再灌注损伤 电针 预处理 心经 小脑顶核 下丘脑外侧区 

分 类 号：R245.97[医药卫生—针灸推拿学]