鸡滑液囊支原体对常用抗菌药的耐药性研究  

Antibacterial Resistance to Common Antibiotics of Mycoplasma synoviae Isolated from Chickens

作　　者：张惠茹 肖霞[1,2] 杨铜 王莉 刘岳龙 王志强 ZHANG Huiru;XIAO Xia;YANG Tong;WANG Li;LIU Yuelong;WANG Zhiqiang(College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou,Jiangsu 225009;Jiangsu Lihua Animal Husbandry Co.,Ltd.,Changzhou,Jiangsu 213168)

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]江苏高校动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009 [3]江苏立华牧业股份有限公司,江苏常州213168

出　　处：《中国家禽》2021年第4期36-42,共7页China Poultry

基　　金：江苏高校优势学科建设工程项目(PAPD)。

摘　　要：为研究常用抗菌药对滑液囊支原体的敏感性及耐药基因的流行分布,试验使用微量肉汤微稀释法确定了截短侧耳素类、大环内酯类、氟喹诺酮类药物对21株鸡滑液囊支原体临床分离株的最小抑菌浓度,并且对相关耐药基因进行探讨。结果显示:1株分离株对截短侧耳素类抗生素耐药(4.76%),7株对泰乐菌素耐药(33.3%),5株对替米考星耐药(23.8%),21株对红霉素、氟喹诺酮类药物均耐药(100%);序列分析显示,对大环内酯类耐药可能与编码核糖体蛋白L4、L22 rpl D(T43C、T167C、A183G)及rpl V(T11C、T67A、C330T)突变相关;对氟喹诺酮类耐药可能与编码DNA拓扑异构酶Ⅱgyr A(T58C、A154G、G400A)、gyr B(A11G、T14A、C124T)等突变,以及编码拓扑异构酶Ⅳpar C(G61A、C149T、C162T)及par E(A122G、C291T、C680T)等突变相关。结果表明:大环内酯类、氟喹诺酮类药物耐药性严重,滑液囊支原体耐药性的产生可能与相关基因处突变有关。研究结果为临床合理使用抗菌药提供了试验依据。To investigate the susceptibility of common antibiotics and the distribution of antimicrobial resistance genesin Mycoplasma synoviae, minimal inhibitory concentration of 21 M. synoviae strains against macrolides, fluoroquinolonesand pleuromutilins were determined by broth microdilution method. Moreover, the genes related to macrolides andfluoroquinolones resistance were analyzed. The results showed that 1 strain exhibited resistance to pleuromutilins, 7 strainswere resistant to tylosin, and 5 strains were resistant to tilmicosin, while all strains exhibited resistance to erythromycinand fluoroquinolones. Through comparing with MS53 standard sequence, resistance to macrolides was mainly related tothe mutation site in rpl D(T43 C, T167 C, A183 G), rpl V(T11 C, T67 A, C330 T) encoding ribosomal protein L4 and L22.Meanwhile, resistance to fluoroquinolones might attribute to the mutation sites of gyr A(T58 C, A154 G, G400 A), gyr B(A11 G, T14 A, C124 T) encoding DNA topoisomerase Ⅱ and par C(G61 A, C149 T, C162 T), par E(A122 G, C291 T,C680 T) encoding topoisomerase Ⅳ. In this study, it′s demonstrated that M. synoviae strains have great resistance tomacrolides and fluoroquinolones, it might be caused by mutations in macrolides and fluoroquinolones resistance relatedgenes. The results provided experimental basis for clinical use of antimicrobial drugs in M. synoviae infection.

关 键 词：滑液囊支原体 大环内酯类抗菌药 氟喹诺酮类抗菌药 单碱基突变位点 

分 类 号：S852.62[农业科学—基础兽医学]