拟南芥蔗糖合成酶基因AtSUS响应非生物胁迫功能分析  

Functional analysis of Arabidopsis thaliana sucrose synthase gene AtSUS in response to abiotic stress

作　　者：胡冰霜 沈阳 才晓溪 王研 陈悦 孙明哲 贾博为 孙晓丽 HU Bingshuang;SHEN Yang;CAI Xiaox;WANG Yan;CHEN Yue;SUN Mingzhe;JIA Bowei;SUN Xiaoli(Crop Stress Molecular Biology Laboratory,College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China)

机构地区：[1]黑龙江八一农垦大学农学院,作物逆境分子生物学实验室,黑龙江大庆163319

出　　处：《植物生理学报》2021年第2期461-472,共12页Plant Physiology Journal

基　　金：黑龙江省留学归国人员择优资助项目;黑龙江八一农垦大学引进人才科研启动计划项目(XYB201902);黑龙江八一农垦大学基础培育计划项目(ZRCPY201902)。

摘　　要：盐碱、干旱等非生物胁迫会降低作物产量,影响作物品质。为挖掘调控植物耐盐碱的关键基因,课题组对拟南芥(Arabidopsis thaliana)突变体进行了大规模筛选,获得了对NaHCO3胁迫敏感的拟南芥突变体atsus4。鉴于蔗糖合成酶(SUS)在蔗糖代谢中的关键作用,本研究系统分析了拟南芥SUS基因(AtSUS)对非生物胁迫的响应。生物信息学分析结果表明,拟南芥SUS家族蛋白序列具有高度保守性。非生物胁迫表达模式分析发现,包括AtSUS4基因在内的多个SUS基因均响应高渗、高盐、干旱和脱落酸胁迫。通过表型分析进一步发现AtSUS4基因的缺失降低了拟南芥对盐、高渗胁迫的耐性,并提高了对脱落酸的敏感性。最后构建了AtSUS4蛋白互作网络,发现AtSUS4与多个糖代谢相关酶存在相互作用,推测AtSUS4通过调节糖的代谢,在非生物胁迫应答过程中发挥作用。上述结果表明,拟南芥SUS家族蛋白序列高度保守,并参与植物多种非生物胁迫应答。Saline–alkaline,drought and other abiotic stresses reduce crop yields and affect its quality.To excavate key genes with saline–alkaline tolerance,a large-scale screening of Arabidopsis thaliana mutants was conducted.Fortunately,we obtained atsus4 exhibiting sensitive to NaHCO3 stress.In view of the vital role of sucrose synthase(SUS)in sucrose metabolism,the roles of A.thaliana SUS genes(At SUSs)in abiotic stress were systematically analyzed.Bioinformatics analysis showed that AtSUS sequences are highly conserved.Further analysis of abiotic stress expression patterns revealed that the transcripts of AtSUS genes,including AtSUS4,are regulated by hyperosmotic,hypersaline,drought and abscisic acid stresses.Subsequently,phenotypic analysis confirmed that the deletion of At SUS4 reduced the tolerance of A.thaliana to hypersaline and hyperosmotic stresses,but increased its sensitivity to abscisic acid stress.At last,an AtSUS4 protein interaction network was generated.In this network,several enzymes related to glucose metabolism were found to interact with AtSUS4,indicating AtSUS4 might respond to abiotic stress by regulating glucose metabolism.In conclusion,our results suggest that AtSUS family proteins share high sequence similarity and function in response to abiotic stress.

关 键 词：拟南芥 蔗糖合成酶 SUS4 逆境胁迫 

分 类 号：Q943.2[生物学—植物学] Q945.78