汉黄芩素缓解高脂饮食喂养ApoE^(-/-)小鼠的动脉粥样硬化和NF-κB介导的动脉炎症反应  

Wogonin alleviates atherosclerosis and NF-κB-mediated inflammatory response in Apo^(E-/-)mice fed with high fat diet

作　　者：韦磊 王绚 陈雄[2] 湛雯[1] 韩望 WEI Lei;WANG Xuan;CHEN Xiong;ZHAN Wen;HAN Wang(Department of Pharmacy,Children's Hospital Affiliated to Nanjing Medical University,Nanjing 210008,China;Department of Endocrinology,the First Affiliated Hospital,Wenzhou Medical University,Wenzhou 325006,China)

机构地区：[1]南京医科大学附属儿童医院药学部,南京210008 [2]温州医科大学附属第一医院内分泌科,温州325006

出　　处：《天然产物研究与开发》2021年第5期750-757,共8页Natural Product Research and Development

基　　金：浙江省自然科学基金(LWY20H20001);“十三五”南京市卫生青年人才培养工程项目(QRX17173)。

摘　　要：为探究汉黄芩素对高脂饮食喂养ApoE^(-/-)小鼠的动脉粥样硬化和炎症的影响,本研究将ApoE^(-/-)小鼠分为正常饮食组、高脂饮食组、高脂饮食+汉黄芩素10 mg/kg组、高脂饮食+汉黄芩素20 mg/kg组,高脂饮食和药物同时给予,12周后收集动脉组织。采用RT-qPCR法检测IL-6、TNF-α、ICAM-1和VCAM-1的mRNA表达;油红O染色和HE染色检测主动脉根部油脂变化和病理变化;免疫荧光染色检测Moma-2和VCAM-1表达;生化试剂盒检测血液中的血脂水平;Western blot法检测动脉的NF-κB的磷酸化和IκB-α的表达变化。结果显示,与高脂饮食组比较,汉黄芩素治疗组动脉粥样硬化斑块面积减少(P<0.05),但血脂水平无变化;汉黄芩素治疗组动脉中单核/单核巨噬细胞浸润,TNF-α、IL-6、ICAM-1和VCAM-1的mRNA表达水平,VCAM-1的蛋白表达水平和NF-κB信号通路的激活均显著降低(P<0.05)。提示汉黄芩素有效缓解高脂饮食喂养诱导的小鼠动脉粥样硬化,并伴随抑制NF-κB介导的动脉炎症。In order to investigate the effect of wogonin on atherosclerosis and arterial inflammation in ApoE^(-/-)mice fed with high fat diet,ApoE^(-/-)mice were divided into normal diet group(ND),high-fat diet group(HFD),high-fat diet+wogonin(10 mg/kg)group(HFD+wogonin 10 mg/kg)and high-fat diet+wogonin(20 mg/kg)group(HFD+wogonin 20 mg/kg).Mice were given high-fat diet and wogonin for 12 weeks and then the arterial tissues were collected.RT-qPCR was used to detect the mRNA expression of IL-6,TNF-α,ICAM-1 and VCAM-1.Immunofluorescence staining was used to detect the expression of Moma-2 and VCAM-1 in the aortic root.Oil red O staining and HE staining were used to detect the changes of the lipid deposition and pathological changes in the aortic root.Biochemical kits were used to detect the serum lipid levels.Western blot was used to detect the phosphorylation of NF-κB and the expression of IκB-α.The results showed that compared with HFD group,the area of atherosclerotic plaque in HFD+wogonin group was decreased(P<0.05),but the serum lipid levels do not change;moreover,monocyte/monocyte macrophage infiltration,the protein expression of VCAM-1,the mRNA expression level of TNF-α,IL-6,ICAM-1 and VCAM-1 and the activation of NF-κB signal pathway in HFD+wogonin group were significantly reduced(P<0.05).These findings indicated that wogonin could effectively alleviate arterial atherosclerosis and inhibit NF-κB-mediated arterial inflammation in ApoE^(-/-)mice induced by high-fat diet.

关 键 词：汉黄芩素 动脉粥样硬化 炎症反应 NF-ΚB 

分 类 号：R966[医药卫生—药理学]