儿童EBV感染与IL-10启动子基因多态性的相关性  

Correlation between IL-10 promoter gene polymorphism and EBV infection in children

作　　者：周雯倩 朱亚非[1] 杨瑜璐 ZHOU Wen-qian;ZHU Ya-fei;YANG Yu-lu(Affiliated Hospital of Hangzhou Normal University,Hangzhou,Zhejiang310015,China;不详)

机构地区：[1]杭州师范大学附属医院儿科,浙江杭州310015 [2]嘉兴市妇幼保健院儿科,浙江嘉兴314001

出　　处：《中华医院感染学杂志》2021年第8期1262-1266,共5页Chinese Journal of Nosocomiology

基　　金：杭州市科技局基金资助项目(20191203B107)。

摘　　要：目的探讨白细胞介素-10(Interleukin-10,IL-10)启动子基因-1082A/G、-819T/C、-592A/C位点多态性与儿童EB病毒(Epstein-Barr virus,EBV)感染易感性及肝功能损害相关性。方法选取杭州师范大学附属医院儿科2019年1月-2019年12月收治EBV感染所致传染性单核细胞增多症(Infectious mononucleosis,IM)患儿87例纳入感染组,其中肝功能损害52例(肝损组),无肝功能损害35例(无肝损组),并选取同期未感染EBV的健康儿童78名为对照组。应用Sanger双脱氧链终止法检测IL-10启动子基因-1082A/G、-819T/C、-592A/C位点的基因多态性。结果IL-10-1082A/G、IL-10-819T/C、IL-10-592A/C位点在感染组与对照组之间基因型及等位基因比较差异无统计学意义;IL-10-1082A/G基因型与等位基因在肝损组及无肝损组之间无统计学差异,IL-10-819T/C的CC基因型频率与IL-10-592A/C的CC基因型频率在肝损组中更高(P<0.05),但两者的等位基因在肝损组及无肝损组中无明显统计学差异;IL-10-819T/C与IL-10-592A/C两个位点基因高度连锁。结论IL-10启动子基因-1082A/G、-819T/C、-592A/C位点多态性与杭州地区儿童EBV易感性无关,IL-10-819T/C位点及IL-10-592A/C位点的CC基因型可能是IM患儿肝功能损害的影响因素之一,但需要更多检验。-819T/C基因型与-592A/C基因型高度连锁。OBJECTIVE To investigate the relationship between polymorphisms of interleukin-10 gene promoter(IL-10-1082 A/G,-819 T/C,-592 A/C)and susceptibility to EB virus infection and liver function damage in children.METHODS A total of 87 children with infectious mononucleosis(IM)caused by EB virus infection in the Department of Pediatrics,affiliated Hospital of Hangzhou normal University from Jan 2019 to Dec 2019 were included in the infection group,including 52 cases of liver function damage(the liver damage group)and 35 cases without liver function damage(the no liver damage group),and 78 healthy children without EBV infection during the same period were recruited as the control group.Detection of polymorphisms of IL-10 gene promoter IL-10-1082 A/G,-819 T/C and-592 A/C by Sanger dideoxy chain termination method.RESULTS There was no significant difference in genotypes and alleles between the infection group and control group in IL-10-1082 A/G,IL-10-819 T/C and IL-10-592 A/C loci.There was no significant difference in IL-10-1082 A/G genotypes and alleles between the liver damage group and non-liver damage group;the CC genotype frequency of IL-10-819 T/C and the CC genotype frequency of IL-10-592 A/C were significantly higher than that in the liver damage group(P<0.05),however,there was no significant difference between the liver damage group and non-liver damage group.The IL-10-819 T/C and IL-10-592 A/C gene loci were highly linked.CONCLUSION Polymorphisms of IL-10 gene promoter IL-10-1082 A/G,-819 T/C and-592 A/C are not associated with EBV susceptibility in children in Hangzhou,and the CC genotypes of IL-10-819 T/C and IL-10-592 A/C loci may be one of the influencing factors for liver function damage in children with IM,which may need more verification.The IL-10-819 T/C and IL-10-592 A/C gene loci are highly linked.

关 键 词：EPSTEIN-BARR病毒 传染性单核细胞增多症 白细胞介素-10 基因多态性 

分 类 号：R725.1[医药卫生—儿科]