不同因素对水溶液中天麻苷元降解速率影响  

Effect of Different Factors on the Degradation Rate of Gastrodigenin in Aqueous Solution

作　　者：邱红燕 周雪[1] 姚淙文[1,3] 刘学 肖婷 廖海伟 胡晓霞 林燕[1] 许乾丽 沈祥春[1] 陶玲 QIU Hongyan;ZHOU Xue;YAO Congwen;LIU Xue;XIAO Ting;LIAO Haiwei;HU Xiaoxia;LIN Yan;XU Qianli;SHEN Xiangchun;TAO Ling(Guizhou Medical University,Guiyang 550025,Guizhou,China;Guizhou Institute for Food and Drug Control,Guiyang 550004,Guizhou,China;Guizhou Institute for Food Control,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学,贵州贵阳550025 [2]贵州省食品药品检验所,贵州贵阳550004 [3]贵州省食品检验检测所,贵州贵阳550004

出　　处：《辽宁中医药大学学报》2021年第4期47-50,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：贵州省科技创新团队项目(黔科合人才团队[2015]4025号);贵州省高层次创新型人才百层次人才项目(贵州省科技厅黔科合人才[2015]4029号);贵州医科大学药学国际科技合作基地资助项目(黔科合平台人才[2017]5802);贵州医科大学博士启动基金(YJ2017-30);贵州省社会攻关计划项目(黔科合[2016]支撑2826);贵州省科技厅联合学术新苗项目(黔科合平台人才[2017]5718);贵阳市科技局联合基金项目(筑科合同[2017]30-16号);贵州省科学技术厅学术新苗培养及创新探索专项(黔科合平台人才[2017]5718)。

摘　　要：目的考察溶液的pH值、磷酸盐缓冲溶液、温度、酸碱盐种类对水溶液中天麻苷元降解速率的影响。方法含量测定的高效液相色谱法(HPLC)条件为流动相乙腈-0.05%磷酸(5∶95,V/V),检测波长为273 nm,柱温35℃,测定天麻苷元在不同条件水溶液中的浓度变化,以剩余药物的百分含量(%)为纵坐标,时间(t)为横坐标,绘制降解动力学曲线,对降解差异显著的条件拟合降解动力学方程。结果天麻苷元在pH 1.2、5.0、11.0及盐酸、碳酸钠、磷酸氢二钠水溶液中稳定性较差,降解反应分别符合一级、零级、三级、零级、零级、三级动力学方程。在pH 4.5、6.8、7.2、9.0水溶液中,浓度为0.01、0.02、0.03、0.04、0.05、0.1、0.2 mol/L的磷酸盐缓冲溶液中,不同温度水溶液中及酒石酸、柠檬酸、氢氧化钠溶液中稳定性较好。结论天麻苷元的水溶液在pH 4.5、6.8、7.2、9.0水溶液,不同浓度的磷酸盐缓冲溶液,不同温度水溶液及酒石酸、柠檬酸、氢氧化钠溶液条件下较稳定。Objective To investigate the effects of pH,phosphate buffer solution,temperature and the types of acids and bases on the degradation rate of gastrodigenin in aqueous solution. Methods The HPLC method was used to determine the concentration of gastrodigenin in aqueous solution under different conditions with acetonitrile-0.05% phosphoric acid(5 ∶ 95,V/V)as mobile phase. The detection wavelength was 273 nm and the column temperature was 35 ℃. The degradation kinetics curve was plotted with the percentage of residual drug(%)as ordinate and time(t)as abscissa. The degradation kinetics equation was fitted to the condition that the degradation difference was significant. Results The stability of gastrodigenin was rather poor in pH 1.2,5.0,11.0,hydrochloric acid solution,sodium carbonate solution,and disodium hydrogen phosphate solution. The gastrodigenin followed first-order,zero-order,thirdorder,zero-order,zero-order and third-order kinetics respectively. The stability of gastrodigenin was good under pH 4.5,6.8,7.2,9.0,phosphate buffer solutions with concentrations of 0.01,0.02,0.03,0.05,0.1 and 0.2 mol/L,different temperatures,tartaric acid solution,citric acid solution and sodium hydroxide solution. Conclusion Gastrodigenin in aqueous solution was stable in pH 4.5,6.8,7.2,9.0,phosphate buffer solutions with different concentrations,different temperatures,tartaric acid solution,citric acid solution and sodium hydroxide solution.

关 键 词：天麻苷元 PH 缓冲溶液 酸碱盐 降解动力学 

分 类 号：R284[医药卫生—中药学]