1株柳珊瑚来源真菌Penicillium chrysogenum中生物碱化合物研究  

Research on the alkaloids from the gorgonian coral-derived fungus Penicillium chrysogenum

作　　者：毛宁 徐伟锋 邵长伦[1] 魏美燕[1] MAO Ning;XU Wei-feng;SHAO Chang-lun;WEI Mei-yan(Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003

出　　处：《中国海洋药物》2021年第2期17-20,共4页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(U1706210,41776141,41606172)资助。

摘　　要：目的对1株南海柳珊瑚来源真菌Penicillium chrysogenum(CHNSCLM-0019)中的生物碱类化合物进行研究。方法综合运用正、反相硅胶柱层析、高效液相色谱(HPLC)及重结晶等方法对真菌粗提物进行分离纯化,并通过现代波谱学方法、单晶衍射及核磁共振变温实验并结合文献对所得化合物进行结构鉴定。结果鉴定了6个苯并二氮杂卓类生物碱1~6,分别为7-methoxycyclopenin (1),cyclopenin (2),7-methoxycyclopeptin(3),(3S)-1, 4-benzodiazepine-2, 5-diones (4),7-methoxydehydrocyclopeptin (5),(+)-cyclopenol (6)。并通过核磁共振变温实验验证了化合物1以互变构象异构体形式存在。结论柳珊瑚来源真菌P. chrysogenum(CHNSCLM-0019)是苯并二氮杂卓类生物碱的重要来源。Objective To study the alkaloids from the gorgonian coral-derived fungus Penicillium chrysogenum(CHNSCLM-0019). Methods The pure compounds were isolated by Silica gel column chromatography, ODS, HPLC and recrystallization. Their structures were determined by modern spectroscopic methods, X-ray, variable temperature NMR, and comparison with previously reported data. Results The structures of six benzodiazepine alkaloids 1~6 were determined as 7-methoxycyclopenin(1), cyclopenin(2), 7-methoxycyclopeptin(3),(3 S)-1, 4-benzodiazepine-2, 5-diones(4), 7-methoxydehydrocyclopeptin(5), and(+)-cyclopenol(6). Compound 1 was determined as tautomorphic isomers. Conclusion The gorgonian coralderived fungus P. chrysogenum was an important resource of benzodiazepine alkaloids.

关 键 词：柳珊瑚共附生真菌 Penicillium chrysogenum 生物碱 阻转异构体 

分 类 号：R931.77[医药卫生—生药学]