Aβ_(1-42)对大鼠脑组织SOD、GSH-Px活性及MDA含量的影响  

Effects of Aβ_(1-42) on the activity of SOD,GSH-Px and the content of MDA in rats brain

作　　者：段文彪[1] 岳嗣凤 陈景锋 张嫄怡 陈雪[1] DUAN Wen-biao;Yue Si-feng;Chen Jing-feng;Zhang Yuan-yi;Chen Xue(Zhaoqing Medical College,Zhaoqing,Guangdong Province 526020 China)

机构地区：[1]肇庆医学高等专科学校,广东肇庆526020 [2]桂林医学院附属医院,广西桂林541004

出　　处：《解剖学研究》2021年第2期133-137,共5页Anatomy Research

基　　金：广东省肇庆市科技创新指导类项目(201804031401);肇庆医学高等专科学校中青年科技基金项目(Zqyq19-003)。

摘　　要：目的探究Aβ1-42寡聚体对大鼠大脑的氧化应激损伤的影响。方法经过Morris水迷宫实验,挑选出逃避潜伏期少于60s的60只实验大鼠随机等分为4组,设为正常组、PBS对照组、Aβ1-42纤维组、Aβ1-42寡聚体组。将Aβ1-42纤维或Aβ1-42寡聚体注射于大鼠右侧脑室制成实验动物模型;PBS对照组采用同样的方法给予注射等量的PBS;正常组不作任何处理。使用可见分光光度计检测大鼠脑组织的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性变化及丙二醛(MDA)的含量水平变化情况。借助于光学显微镜观察苏木精-伊红(HE)染色的各组大鼠海马组织的形态学变化。结果与正常组及PBS组比较,Aβ1-42纤维组和Aβ1-42寡聚体组大鼠皮质MDA含量(分别为7.826±1.696、11.247±1.948)及海马MDA(分别为9.818±1.900、15.090±2.425)含量增加(P<0.05),皮质SOD(分别为29.829±6.340、18.649±6.070)和海马SOD(分别为51.097±8.381、31.634±8.680)活性下降(P<0.05),皮质GSH-Px(分别为133.113±17.506、79.503±19.936)和海马GSH-Px(分别为154.819±23.688、102.800±28.332)活性下降(P<0.05),其中,以Aβ1-42寡聚体组的变化更明显。HE染色可知,Aβ1-42纤维体及Aβ1-42寡聚体可使大鼠大脑海马区的神经元损伤,其中,Aβ1-42寡聚体对神经元的毒性损伤作用更严重。结论 Aβ1-42纤维、Aβ1-42寡聚体都可通过氧化应激损伤引起大鼠脑组织神经元的损伤,以Aβ1-42寡聚体氧化损伤作用更大。Objective To investigate the effects of Aβ1-42 on oxidative stress damage in rats brain.Methods After screening with Morris water maze,60 rats with escape latency less than 60s were randomly assigned equally into four groups.Namely,the normal group,PBS control group,Aβ1-42 fiber group,Aβ1-42oligomers group.The rats in Aβ oligomers and Aβfiber group were infused Aβ1-42oligomers or Aβ1-42fiber into right cerebral ventricle to make experimental animal models,PBS control group received the same volume of PBS,normal group received no treatment.Visible spectrophotometer assay was used to detect the activity of SOD,GSH-Px and the content of MDA.Used HE staining and optical microscope to observe the morphological changes of rats’hippocampus.Results Compared with normal and PBS control group,the content of MDA in cortex(7.826±1.696),(11.247±1.948)and hippocampus(9.818±1.900),(15.090±2.425)were increased in Aβ1-42fiber group and Aβ1-42oligomer group(P<0.05),the activity of SOD in cortex(29.829±6.340),(18.649±6.070)and hippocampus(51.097±8.381),(31.634±8.680)were decreased in Aβ1-42fiber group and Aβ1-42oligomers group(P<0.05),Aβ1-42fiber group and Aβ1-42oligomers group’s GSH-Px activity in cortex(133.113±17.506),(79.503±19.936)and hippocampus(154.819±23.688),(102.800±28.332)were decreased(P<0.05).The above-mentioned changes in Aβ1-42oligomers group were more prominent than in Aβ1-42fiber group.HE staining shows that Aβ1-42fiber and Aβ1-42oligomers can damage neurons in the hippocampus of the rat brain,in which Aβ1-42oligomers has much greater neuronal toxicity.Conclusion Aβ1-42fiber and Aβ1-42oligomers can all lead to oxidative stress damage in rats’cerebral tissue,cause neuron damage,but Aβ1-42oligomers has larger oxidative stress damage.

关 键 词：阿尔茨海默病 Aβ1-42纤维 Aβ1-42寡聚体 氧化应激 

分 类 号：R749.16[医药卫生—神经病学与精神病学]