大鼠阴道重建动物模型的构建  

The establishment of an animal model for vaginal reconstruction in rats

作　　者：张宁[1] 秦锡静 张志强[3] 张敬坤[3] 黄向华[3] 朱玉林 ZHANG Ning;QIN Xi-jing;ZHANG Zhi-qiang;ZHANG Jing-kun;HUANG Xiang-hua;ZHU Yu-lin(Department of Reproductive Medicine, the Fourth Hospital of Shijiazhuang City, Hebei Province, Shijiazhuang 050011, China;Department of Obstetrics and Gynecology, the Xiangya Changde Hospital, Hunan Province, Changde 415000, China;Department of Obstetrics and Gynecology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China)

机构地区：[1]河北省石家庄市第四医院生殖医学中心,河北石家庄050011 [2]湖南省常德市湘雅常德医院妇产科,湖南常德415000 [3]河北医科大学第二医院妇科,河北石家庄050000

出　　处：《河北医科大学学报》2021年第5期501-504,F0003,共5页Journal of Hebei Medical University

基　　金：国家自然科学基金资助项目(81471418);河北省医学科学研究重点课题计划(20201389)。

摘　　要：目的构建大鼠阴道重建动物模型。方法手术切除雌性大鼠阴道,将小肠黏膜下基质(small intestinal submucosa,SIS)置入切除后形成的腔穴内重建大鼠阴道。分别于术后14 d、1个月、3个月随机处死6只大鼠,取出阴道全层检测重建阴道组织中上皮组织、平滑肌组织及神经组织的的生长情况,取4只大鼠于3个月时处死用组织浴槽的方法进行功能学检测,即给予不同的电场刺激(electrical field stimulation,EFS),检测重建阴道组织张力的变化情况。结果大鼠阴道重建手术能很好的模拟无阴道患者的阴道成形术。重建阴道14 d时质地比较韧,没有弹性,随时间延长质地逐渐变软,至3个月时明显变软,并且有一定的弹性。重建阴道镜检:14 d时可见有部分上皮组织从阴道入口向上爬行,伴有较多炎细胞浸润,1个月时上皮组织能覆盖整个阴道,3个月时上皮组织更加成熟。新生上皮组织表达广谱角蛋白AE1/AE3;1个月时重建阴道组织中有少量组织表达α平滑肌肌动蛋白(α-smooth muscle actin,α-SMA),提示平滑肌的再生,3个月时α-SMA表达增多局部形成束状;直到3个月时重建阴道组中才偶见蛋白基因产物9.5(protein gene product 9.5,PGP9.5)阳性的神经组织。组织浴槽实验中在电场刺激下重建阴道组织有轻微的收缩反应,收缩的图形和变化趋势与正常阴道类似,但收缩强度明显小于正常阴道。结论用大鼠构建阴道重建模型操作简便,可用于评估多种组织材料用于阴道重建的效果,为进一步构建更好的重建阴道奠定了基础。Objective To establish an animal model for vagina reconstruction in rats.Methods The vagina of female rats was surgically removed,and the small intestinal submucosal(SIS)matrix was placed in the cavity formed after the resection to reconstruct the vagina of the rat.Six rats were randomly sacrificed at 14 days,1 month,and 3 months after the operation.The entire vagina was taken out to detect the growth of epithelial tissue,smooth muscle tissue and nerve tissue in the reconstructed vaginal tissue.Four rats were sacrificed at 3 months for functional evaluation by means of tissue bath assay.Different electrical field stimulation(EFS)was used to detect changes in the tension of the reconstructed vaginal tissue.Results The rat model for vagina reconstruction was successfully established.At 14 days after reconstruction,the texture of neovagina was relatively tough and inelastic,which,however,gradually became softer with time.It became significantly softer and had a certain degree of elasticity at 3 months.Colposcopy after reconstruction revealed that at 14 days,some epithelial tissues could be seen crawling up from the entrance of the neovagina,accompanied by more inflammatory cell infiltration;the epithelial tissue could cover the entire neovagina at 1 month,and the epithelial tissue was more mature at 3 months.Regenerate epithelial tissues expressed AE1/AE3,and a small amount of tissues in neovaginal tissues expressedα-SMA at one month after reconstruction,suggesting the regeneration of smooth muscles.At 3 months,the expression ofα-SMA increased and formed bundles.PGP9.5-positive nerve tissue was occasionally seen in the vagina reconstruction group at 3 months.In the tissue bath assay,slight contraction of neovagina was found under the EFS.The contraction pattern and change trend were similar to those of normal vagina,but the contraction intensity was significantly smaller than normal vagina.Conclusion The rat model for vagina reconstruction was readily established.It can be used to evaluate the effects of various t

关 键 词：阴道疾病 修复外科手术 组织工程 

分 类 号：R711.21[医药卫生—妇产科学]