新疆野苹果优选无性系DNA指纹图谱构建及亲缘关系鉴定  

Construction of DNA fingerprint and identification of genetic relationship of Xinjiang wild apple clones

作　　者：李昕蔓 庞丁玮 柳俊明 王立成 李清泉 张军[1] LI Xinman;PANG Dingwei;LIU Junming;WANG Licheng;LI Qingquan;ZHANG Jun(College of Forestry, Hebei Agricultural University, Baoding 071000, China;Hebei Wuling Mountain National Nature Reserve Administration, Xinglong 067300, China;Nursery Farm in Mancheng District of Baoding City, Mancheng 072150, China)

机构地区：[1]河北农业大学林学院,河北保定071000 [2]河北雾灵山国家级自然保护区管理局,河北兴隆067300 [3]保定市满城区苗圃场,河北满城072150

出　　处：《林业与生态科学》2021年第2期138-143,共6页Forestry and Ecological Sciences

基　　金：河北农业大学科研发展基金计划(JY2018006);国家重点研发计划课题(2016YFC0503106)。

摘　　要：新疆野苹果是苹果属重要的抗逆育种资源,观赏价值较高,是园林绿化的优良树种。以18个新疆野苹果无性系为研究对象,基于SSR分子标记技术对其进行分子鉴定,并构建了DNA指纹图谱。结果表明:13对SSR引物共扩增出62条条带,每对引物扩增条带数在3~6条之间,平均每对引物扩增条带4.77条,每对引物的多态位点百分比为100%,各引物的多态性信息含量PIC值的变化范围为0.512~0.879。在遗传距离0.566处,18个新疆野苹果无性系被分成4类,第一类为M1、M14、M13、M16、M3、M4、M6等7个无性系;第二类为M151个无性系;第三类为M2、M5、M9、M11、M12等5个无性系;第四类为M7、M17、M18、M8、M10等5个无性系。基于SSR扩增结果,构建18个新疆野苹果无性系的DNA指纹图谱及二维码。Xinjiang wild apple is an important stress-resistant breeding resource of Malus with has high ornamental value and is an excellent tree species for landscape greening.In this study,18 Xinjiang wild apple clones were identified by SSR molecular marker technology,and the DNA fingerprint was constructed.The results showed that a total of 62 bands were amplified by 13 pairs of SSR primers.The number of bands amplified by each pair of primers ranged from 3 to 6.The average number of bands amplified by each pair of primers was 4.77.The percentage of polymorphic loci per pair of primers was 100%.The PIC value of polymorphic information content of each pair of primers ranged from 0.512 to 0.879.18 Xinjiang wild apple clones were classified into four groups at genetic distance of 0.566.The first category is 7 clones:M-1,M-14,M-13,M-16,M-3,M-4,M-6;the second category is 1 clone:M-15;the third category is 5 clones:M-2,M-5,M-9,M-11,M-12;the fourth category is 5 clones:M-7,M-17,M-18,M-8,M-10.Based on the results of SSR amplification,DNA fingerprints and two-dimensional codes of 18 wild apple clones were constructed.

关 键 词：新疆野苹果 SSR分子标记 聚类分析 DNA指纹图谱-3 

分 类 号：S661.1[农业科学—果树学]