大黄不同炮制品的大承气汤对血清内毒素、NO和TNF-α含量变化的影响  

Effect of Dachengqi Decoction(大承气汤)of Different Dahuang(Rhei Radix Et Rhizoma)Products on Contents of Endotoxin,NO and TNF-αin Serum

作　　者：魏江存 秦祖杰 谢臻[2] 戴铭 王成龙[1] 李耀华 马秀梅[1] 韩倩 唐云丽 WEI Jiangcun;QIN Zujie;XIE Zhen;DAI Ming;WANG Chenglong;LI Yaohua;MA Xiumei;HAN Qian;TANG Yunli(Guangxi International Zhuang Medical Hospital,Nanning 530201,Guangxi,China;Guangxi University of Chinese Traditional Medicine,Nanning 530200,Guangxi,China;Guangxi Key Lalwratory of Chinese Medicine Foundation Research,Guangxi University of Chinese Medicine,Nanning 530200,Guangxi,China)

机构地区：[1]广西国际壮医医院,广西南宁530201 [2]广西中医药大学,广西南宁530200 [3]广西中医药大学广西中医基础研究重点实验室,广西南宁530200

出　　处：《中华中医药学刊》2021年第3期64-68,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81360524);国家中医药管理局科研三级实验室中(壮)药化学与质量分析实验室(国中医药发2009[21]号);广西中医药大学青年基金(2019QN036);广西中医药大学2019-2021年广西一流学科建设开放课题资助项目青年基金(2019XK089);广西中医基础研究重点实验室开放研究课题(KJT1701205);广西高校中青年教师科研基础能力提升项目(2019KY0341)。

摘　　要：目的研究大黄不同炮制品配伍后的大承气汤对血清内毒素、NO和TNF-α含量变化的影响。方法将165只实验动物随机分为大黄不同炮制品配伍后的大承气汤组(6 g/kg、10 g/kg)、阳性对照组、空白对照组及模型组,按容积20 mL/kg的剂量给药后,测定血清内毒素、一氧化氮和肿瘤坏死因子含量,比较大黄不同炮制品配伍后的大承气汤对小鼠血清内毒素、一氧化氮和肿瘤坏死因子含量的影响。结果大黄不同炮制品的大承气汤组都能增加ABP小鼠血清NO含量,但与其它大承气汤组相比,炭DCQT组增加ABP小鼠血清NO含量不明显。大黄不同炮制品的大承气汤组都能降低ABP小鼠血清内毒素和TNF-α含量,但与其它大承气汤组相比,炭DCQT组降低ABP小鼠血清内毒素和TNF-α含量不明显。结论大黄不同炮制品的大承气汤对内毒素、NO和TNF-α的含量均有不同程度的影响,这些变化与大黄不同炮制品配伍的大承气汤作用相对应,且大黄蒽醌类成分含量的变化在一定程度上对大承气汤的功效存在着一定的影响和共性关系。Objective To study the effects of Dachengqi Decoction(大承气汤)on the levels of endotoxin,NO and TNF-αin serum after compatibility of different processed products of Dahuang(Rhei Radix Et Rhizoma).Methods A total of 165 experimental animals were randomly divided into Dachengqi Decoction groups(6 g/kg,10 g/kg),positive control group,blank control group and model group after the compatibility of different Dahuang(Rhei Radix Et Rhizoma)products.The volume was 20 mL/kg.After the drug was administered,the contents of endotoxin,NO and TNF-αin serum were measured,and the effects of Dachengqi Decoction combined with different processed products of Dahuang(Rhei Radix Et Rhizoma)on endotoxin,NO and TNF-αin mice were compared.Result The Dachengqi Decoction groups of different Dahuang(Rhei Radix Et Rhizoma)products could increase the NO content in serum of ABP mice.But compared with other Dachengqi Decoction groups,the NO content in serum of ABP mice was not obvious in the Dachengqi Decoction with Dahuangtan(charred Rhei Radix Et Rhizoma)group.The Dachengqi Decoction groups of different Dahuang(Rhei Radix Et Rhizoma)products can reduce the serum contents of endotoxin and TNF-αof ABP mice.But compared with other Dachengqi Decoction groups,the serum levels of endotoxin and TNF-αin ABP mice were not obviously reduced in Dachengqi Decoction with Dahuangtan(charred Rhei Radix Et Rhizoma)group.Conclusion Different processed products of Dahuang(Rhei Radix Et Rhizoma)in Dachengqi Decoction have different effects on the contents of endotoxin,NO and TNF-α.These changes correspond to the effect of Dachengqi Decoction in the compatibility of different processed products of Dahuang(Rhei Radix Et Rhizoma),and the contents changes of Dahuang(Rhei Radix Et Rhizoma)anthraquinones to a certain extent have a certain influence and commonality on the efficacy of Dachengqi Decoction.

关 键 词：大黄 大承气汤 炮制品 内毒素 一氧化氮 肿瘤坏死因子 

分 类 号：R285.5[医药卫生—中药学]