DNA条形码技术在淫羊藿属植物分类鉴定中的可行性分析  

Taxonomy and Identification Analysis of Epimedium(Berberidacae)Applying DNA Barcoding

作　　者：严福林 孙庆文 任得强 徐文芬 魏升华 何顺志 YAN Fulin;SUN Qingwen;REN Deqiang;XU Xenfen;WEI Shenghua;HE Shunzhi(Department of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,Guizhou,China;State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medcial University,Guiyang 550000,Guizhou,China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025 [2]贵州医科大学药用植物功效与利用国家重点实验室,贵州贵阳550000

出　　处：《中华中医药学刊》2021年第3期69-72,I0015,I0016,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81160506);国家自然科学基金喀斯特联合项目(U1812403-2-1);国家中医药管理局项目(国中医药科技中药2014117号);贵州省国内一流学科建设项目(GNYL[2017]008);贵州省普通高等学校工程研究中心项目(黔教合KY字[2017]018);贵州省优秀青年科技人才项目(黔科合平台人才[2019]5658);贵州省教育厅项目(黔教合KY字[2017]179)。

摘　　要：目的拟探讨基于叶绿体基因片段psbA-trnH与matK序列的DNA条形码技术在淫羊藿属中的分类与鉴定中的可行性。方法选用不同来源的34个种,共计84份材料,对其psbA-trnH与matK序列片段进行PCR扩增、测序,分析其序列组成和结构特点,应用Kimura双因素模型(Kimura-2-parameter,K-2-P)计算遗传距离,同时应用邻接法(Neighbor-Joining,N-J)构建系统发育树。结果PsbA-trnH序列在34种淫羊藿属材料中的通用性较matK好;通过K-2-P模型评估,psbA-trnH鉴定成功率为36.7%,matK鉴定成功率为24.2%,2条序列遗传距离均存在一定gap;N-J进化树显示,matK序列N-J系统进化树鉴定效率为17.1%,psbA-trnH序列N-J系统进化树鉴定效率为25.7%。结论本研究丰富了淫羊藿属植物的分类鉴定资料,但在淫羊藿属中的分类与鉴定中,应用基于叶绿体基因片段psbA-trnH与matK序列的DNA条形码技术仍具有一定局限性。Objective This study intends to explore the feasibility of taxonomy and identification in Epimedium(Berberidacae)applying DNA Barcoding technology based on chloroplast gene fragments psbA-trnH and matK sequences.Methods A total of 84 materials from 34 species of different sources were selected to amplify and sequence the psbA-trnH and matK sequence fragments by PCR.Their sequence composition and structure were analyzed.The genetic distance was calculated by Kimura two-factor model(K-2-P),and the phylogenetic tree was constructed by Neighbor-Joining(N-J).Results The results showed that the generality of PsbA-trnH sequence in 34 species of Epimedium was better than that of matK.According to the K-2-P model,the identification success rate of psbA-trnH was 36.7%,that of matK was 24.2%,and there were some gaps between the two sequences.The N-J phylogenetic tree showed that the identification efficiency of matK sequence was 17.1%,and that of psbA-trnH sequence was 25.7%.Conclusion It indicated that this study enriched the taxonomic identification data of Epimedium,but applying DNA barcoding based on chloroplast gene fragments psbA-trnH and matK sequences in taxonomy and identification in Epimedium(Berberidacae)in this study,was quite limited.

关 键 词：淫羊藿属 PSBA-TRNH MATK 分类鉴定 DNA条形码 

分 类 号：R284.1[医药卫生—中药学]