甜菜NAC转录因子家族BvNAC46基因的RNAi载体构建  

Construction of RNAi Vector of NAC-like Transcription Factors BvNAC46 Gene from Sugar Beet

作　　者：李国龙[1] 吴海霞 张少英[1] 孙亚卿[1] Li Guolong;Wu Haixia;Zhang Shaoying;Sun Yaqing(Agricultural College,Inner Mongolia Agricultural University,Hohhot,010019;Inner Mongolia Research Institute for Water Conservancy,Hohhot,010020)

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019 [2]内蒙古自治区水利科学研究院,呼和浩特010020

出　　处：《分子植物育种》2021年第8期2579-2587,共9页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31760414,31360355)资助。

摘　　要：NAC转录因子家族成员在调控植物生长发育、参与植物生物和非生物胁迫等方面发挥着重要作用。本研究以抗旱甜菜(Beta vulgaris L.)幼苗叶片为材料,提取叶片总RNA并反转录cDNA,通过PCR方法扩增获得甜菜NAC转录因子家族成员BvNAC46基因的RNAi靶片段,利用中间载体pBSK作为媒介,采用传统的"酶切-连接"法构建了含有CaMV 35S启动子、BvNAC46基因片段反向重复序列的RNAi (RNA interference)植物表达载体pCambia2301ky-BvNAC46-RNAi,为进一步鉴定BvNAC46基因在甜菜抗旱中的功能及其作用机制奠定基础。The NAC transcription factor family play an important role in regulating plant growth, development and biological and abiotic stress. In this study, the leaves of sugar beet(Beta vulgaris L.) seedling was used as the material, the total RNA was extracted and reverse transcribed into cDNA. The RNAi target fragment of BvNAC46 gene in sugar beet was obtained by PCR amplification. Based on the intermediate vector pBSK, the RNAi(RNA interference) plant expression vector pCambia2301 ky-BvNAC46-RNAi with inverted repeats of BvNAC46 driven by the promoter CaMV 35 S was successfully constructed by traditional restriction enzyme digestion and ligation methods, which might be helpful for the study of drought function and its mechanism in future.

关 键 词：甜菜 NAC转录因子 RNA干扰 抗旱 

分 类 号：S566.3[农业科学—作物学]