落叶松针叶直接不定芽发生体系的建立  

Direct Regeneration of Adventitious Bud from Needles of Larix

作　　者：王可佳 闫学彤 郑珂媛 陈海霞[1] 朱木兰 Wang Kejia;Yan Xuetong;Zheng Keyuan;Chen Haixia;Zhu Mulan(Hunan Mid-subtropical Quality Plant Breeding and Utilization Engineering Technology Research Center,College of Horticulture,Hunan Agricultural University,Changsha,410128;Chinese Academic Scientific Center for Excellence in Molecular Plant Science,Shanghai,200032;Shanghai Key Laboratory of Plant Functional Genomics and Resources,Shanghai Chenshan Plant Science Research Center,Shanghai Chenshan Botanical Garden,Shanghai,201602)

机构地区：[1]湖南农业大学园艺学院,湖南省中亚热带优质花木繁育与利用工程技术研究中心,长沙410128 [2]中国科学院分子植物科学卓越创新中心,上海200032 [3]上海辰山植物园,中国科学院上海辰山植物科学研究中心,上海市资源植物功能基因组学重点实验室,上海201602

出　　处：《分子植物育种》2021年第8期2719-2724,共6页Molecular Plant Breeding

基　　金：国家科技重大专项项目(2018ZX08020-003)资助。

摘　　要：本研究以落叶松针叶为起始外植体,开展直接不定芽诱导、伸长及不定根诱导等研究,建立了落叶松针叶直接不定芽发生体系,为该物种基因工程育种、基因编辑及优质种苗繁育等提供了全新途径。结果表明,DCR+2 mg/L 6-BA+0.2 mg/L NAA为不定芽诱导的较佳配方,不定芽诱导率达66.33%,平均芽数80个/外植体;伸长分为DCR+0.1 mg/L 6-BA+0.01 mg/L NAA和DCR+0.1 mg/L 6-BA+0.01 mg/L NAA+2 g/L活性炭两个步骤进行,不定芽伸长率达到60%,平均芽苗长度为2.57 cm;DCR+3 mg/L IBA+0.02 mg/L烯效唑为较佳的不定根诱导培养配方,不定根诱导率达到53.33%,移栽成活率达到87.48%。本研究可为落叶松基因工程育种、基因编辑及优质种苗繁育等研究与应用提供基础。In this study, larix needles were used as the initial explants to carry out direct adventitious bud induction, elongation and adventitious root induction, and established a direct adventitious bud generation system for larix needles, which provided a new way for genetic engineering breeding, gene editing and high-quality seedling breeding of this species. The results showed that DCR +2 mg/L 6-BA +0.2 mg/L NAA was a better formula for adventitious bud induction, the induction rate of adventitious buds was 66.33%, and the average bud number was80 per explant. The elongation was divided into DCR+0.1 mg/L 6-BA+0.01 mg/L NAA and DCR+0.1 mg/L 6-BA+0.01 mg/L NAA+2 g/L AC, the elongation rate of adventitious shoots reached 60%, and the average shoot length was 2.57 cm. DCR+3 mg/L IBA+0.02 mg/L sacroconazole was a better adventitious root induction formula, the adventitious root induction rate reached 53.33%, and the transplant survival rate reached 87.48%. This research can provide a foundation for the research and application of larch genetic engineering breeding, gene editing and high-quality seedling breeding.

关 键 词：落叶松 针叶 离体培养 直接再生 

分 类 号：S791.22[农业科学—林木遗传育种]