外源6-BA和BR对干旱胁迫下水稻分蘖期光合色素含量及抗氧化系统的影响  

Effects of Exogenous 6-BA and BR on Photosynthetic Pigment Content and Antioxidant System of Rice at the Tillering Stage under Drought Stress

作　　者：杨喆 唐才宝 钱婧雅 周伟江 陈光辉[1] 王悦[1,2] Yang Zhe;Tang Caibao;Qian Jingya;Zhou Weijiang;Chen Guanghui;Wang Yue(College of Agronomy,Hunan Agricultural University,Changsha,410128;Rice and Rapeseed Disease Resistance Breeding Key Laboratory of Hunan Province,College of Agronomy,Hunan Agricultural University,Changsha,410128)

机构地区：[1]湖南农业大学农学院,长沙410128 [2]湖南农业大学农学院,水稻油菜抗病育种湖南省重点实验室,长沙410128

出　　处：《分子植物育种》2021年第8期2733-2739,共7页Molecular Plant Breeding

基　　金：国家重点研发计划(2017YFD0301501);国家自然科学基金(31971923,31301650);湖南省教育厅科学研究重点项目(19A220)共同资助。

摘　　要：为明确6-苄氨基嘌呤(6-BA)和油菜素内酯(BR)对干旱胁迫下水稻分蘖期生理特性的调控机制,本研究以三系杂交中熟晚稻品种‘H优518’为试验材料,通过喷施25 mg/L 6-BA和BR溶液,研究其对干旱胁迫下水稻分蘖期光合色素含量、抗氧化酶活性、丙二醛含量及可溶性蛋白含量等的影响。结果表明,随着干旱胁迫加剧,水稻分蘖期生长受到明显抑制。喷施6-BA和BR溶液后,在干旱胁迫第9天的水稻分蘖期叶片总叶绿素含量和类胡萝卜素含量相比对照分别显著增加28.1%~32.66%和25.27%~27.47%,而抗氧化酶活性也受到不同程度的促进,丙二醛含量降低了19.99%~34.53%,可溶性蛋白含量增加了13.72%~23.04%,并且喷施6-BA溶液的整体效果要好于BR溶液。由此可见,叶面喷施6-BA和BR能有效缓解干旱胁迫对水稻分蘖期生理特性的影响,对水稻抗旱栽培具有重要意义。The purpose of this study was to elucidate the mechanism of 6-Benzylaminopurine(6-BA) and brassinosteroids(BR) on physiological characteristics of rice at the tillering stage under drought stress. The effects of spraying 25 mg/L 6-BA and BR solution on photosynthetic pigment content, antioxidant enzyme activity, malondialdehyde content and soluble protein content in tillering stage of rice under drought stress were studied with three-line hybrid medium mature late rice variety ’H you 518’ as experimental material. The results showed that the growth of rice at the tillering stage is obviously inhibit with increasing drought stress. After spraying 6-BA and BR solution, the total chlorophyll content and carotenoid content of rice leaves at the tillering stage increased by28.1%~32.66% and 25.27%~27.47% respectively compared with the control on the 9 th day of drought stress. The activities of antioxidant enzymes were also promoted to some extent. The content of malondialdehyde decreased by 19.99%~34.53%, and the content of soluble protein increased by 13.72%~23.04%. The effect of spraying 6-BA solution is better than that of BR solution. It can be seen that spraying 6-BA and BR on the leaf can effectively alleviate the effects of drought stress on the physiological characteristics of rice at tillering stage, which is of great significance for rice drought resistant cultivation.

关 键 词：水稻 分蘖期 干旱胁迫 光合色素 抗氧化系统 

分 类 号：S511[农业科学—作物学]