外源2,4-表油菜素内酯对盐胁迫下对水稻幼苗生理特性的影响  

Effects of Exogenous 2,4-Epibrassinolide on Physiological Characteristics of Rice Seedlings Under Salt Stress

作　　者：安辉 盛伟 于玉凤 张露倩 曾红丽 陈光辉[1] An Hui;Sheng Wei;YuYufeng;Zhang Luqian;Zeng Hongli;Chen Guanghui(College of Agronomy,Hunan Agricultural University,Changsha,410128)

机构地区：[1]湖南农业大学农学院,长沙410128

出　　处：《分子植物育种》2021年第8期2740-2746,共7页Molecular Plant Breeding

基　　金：国家重点研发计划“粮食丰产增效科技创新”重点专项(2018YFD0301005,2016YFD0300509)资助。

摘　　要：为探讨外源2,4-表油菜素内酯(EBR)对盐胁迫下水稻幼苗生理特性的影响。以水稻品种‘湘早籼45号’为试验材料,在盐胁迫下喷施不同浓度外源EBR,研究其对水稻幼苗光合色素及抗氧化系统的影响。结果表明:盐胁迫能显著抑制水稻幼苗光合色素的合成,并对抗氧化系统造成损伤。1μmol/L EBR溶液能有效缓解盐胁迫下水稻幼苗光合色素含量的降低,在盐胁迫24 h时最为明显,其叶绿素a、类胡萝卜素和总叶绿素含量相比对照分别提高51.77%、52.35%、57.62%。同时,1μmol/L EBR溶液喷施能显著提高盐胁迫24 h时的叶片SOD、CAT活性和根系CAT活性,以及盐胁迫恢复后(72 h)的根系SOD活性和叶片POD活性,相比CK2增加了2.91%~24.86%,并且能有效降低MDA含量的积累。因此,在生产上,喷施1μmol/L EBR溶液能有效缓解盐胁迫下水稻幼苗受到的损伤。本研究为盐胁迫下EBR调控水稻幼苗耐盐生理调控机制提供了理论基础。In order to study the effect of exogenous 2,4-epibrassinolide(EBR) on physiological characteristics of rice seedlings under salt stress. The effects of different concentrations of exogenous EBR on photosynthetic pigment and antioxidant system of rice seedlings were studied by spraying the rice variety ’Xiangzhuoxian 45’ under salt stress. The results showed that salt stress could significantly inhibit the synthesis of photosynthetic pigment in rice seedlings and resist the damage of oxidation system. 1 μmol/L EBR solution could effectively alleviate the decrease of photosynthetic pigment content of rice seedlings under salt stress, which was the most obvious at 24 hours of salt stress. The contents of chlorophyll a, carotenoid and total chlorophyll were increased by 51.77%,52.35% and 57.62% respectively compared with the control. Spraying 1 μmol/L EBR solution could significantly improve the SOD, CAT activity of leaves and CAT activity of roots at 24 h of salt stress. The SOD activity of roots and POD activity of leaves after salt stress recovery(72 h) increased by 2.91%~24.86% compared with CK2,which could effectively reduce the accumulation of MDA content. Therefore, spraying 1 μmol/L EBR solution could effectively alleviate the damage of rice seedlings under salt stress in production. This study provided a theoretical basis for the regulation mechanism of EBR on salt tolerance of rice seedlings under salt stress.

关 键 词：水稻 2 4-表油菜素内酯 盐胁迫 光合色素 抗氧化系统 

分 类 号：S511[农业科学—作物学]