56℃30 min灭活病毒方法对输血检验的影响  

Effect of sample processing at 56℃ for 30 min on routine examination in Department of Blood Transfusion

作　　者：展丙香 程艳红 刘春秋[1] 杨鹏[1] 卞茂红[1] ZHAN Bingxiang;CHENG Yanhong;LIU Chunqiu;YANG Peng;BIAN Maohong(Department of Blood Transfusion,the First Affilia­ted Hospital of Anhui Medical University,Heifei 230022,China)

机构地区：[1]安徽医科大学第一附属医院输血科,安徽合肥230022

出　　处：《中国输血杂志》2021年第4期346-349,共4页Chinese Journal of Blood Transfusion

摘　　要：目的探讨标本56℃30 min加热处理后对输血科常规检验的影响。方法收集本院临床科室送检的交叉配血标本40人份,每份标本均分为二,56℃30min加热处理前后标本的ABO血型检测采用手工法和卡式法(凝胶卡和玻璃珠卡),抗体效价的检测采用凝聚胺法,交叉配血采用抗球蛋白卡式法,通过卡方检验和秩和检验来比较2组数据(加热处理前后)的差异。结果实验组与对照组比较,手工试管法测出率为100%(40/40),凝胶卡和玻璃珠卡血型检出率为37.5%(15/40)和80%(32/40)(P<0.01);对照组和实验组血型效价几何均数分别为抗-A IgM型(14.936,10.556)、IgG型(3.563,4.489),抗B IgM型(9.849,10.556)、IgG型(5.039,4.489)(P>0.05);两组标本采用凝聚胺法与相应献血者标本交叉配血,相合率均为100%(40/40),抗球蛋白卡式法则分别为100%和25%(10/40)(P<0.01);实验组其他30例标本次侧均有弱凝集。结论 56℃加热30min灭活病毒的处理对试管法血型鉴定、抗体效价及凝聚胺法交叉配血结果影响甚小,同时又减少了输血科工作人员的职业暴露。Objective To investigate the effect of sample processing at 56℃ for 30 min on routine examination in Department of Blood Transfusion. Methods A total of 40 cross matched blood samples submitted by clinical departments of our hospital, were collected, and each sample was equally divided into two. Before and after heating at 56℃ for 30 min, the ABO blood group was detected by manual method and card method(gel card and glass beadle card), antibody titer was detected by coagulant method, and cross-matching was conducted by anti-globulin card method. Chi-square test and Wilcoxon signed rank test were used to compare the differences between the two groups(before and after heating treatment). Results The blood group detection rates of the experimental group were 100%(40/40), 37.5%(15/40) and 80%(32/40) by manual test tube method, gel card and glass beads card, respectively, P<0.01. The geometric mean of blood antibody titers in the control group and the experimental group were anti-A IgM(14.936,10.556) and IgG(3.563,4.489), anti-B IgM(9.849,10.556) and anti-G(5.039,4.489)(respectively, P>0.05). The matching rate of two groups of samples, cross-matched with corresponding donor samples, was both 100%(40/40) by coagulant method, and 100%(40/40) vs 25%(10/40) respectively by the antiglobulin card method(P<0.01). The other 30 samples in the experimental group presented weak agglutination in the secondary side. Conclusion The treatment of virus inactivation at 56℃ for 30 min has little effect on blood group identification by test tube method, antibody titer and cross-matching by coagulant method, and reduceds the occupational exposure of staff in Blood Transfusion Department.

关 键 词：病毒灭活 加热法 输血科常规检验 血型 血型抗体 交叉配血 

分 类 号：R457.11[医药卫生—治疗学] R373[医药卫生—临床医学]