南京地区血小板捐献者HPA-1-29bw基因分型检测  

Genotyping of HPA-1-29bw in platelet donors in Nanjing, China

作　　者：张若洋[1] 陈蓉[1] 刘太香[1] 蔡莉[1] 梁文飚[1] ZHANG Ruoyang;CHEN Rong;LIU Taixiang;CAI Li;LIANG Wenbiao(Jiangsu Province Blood Center,Nanjing 210042,China)

机构地区：[1]江苏省血液中心,江苏南京210042

出　　处：《中国输血杂志》2021年第4期349-353,共5页Chinese Journal of Blood Transfusion

基　　金：江苏省社会发展科技计划(BE2017757);江苏省卫生健康委员会科技项目(BE2017757);江苏省卫生健康委员会科技项目(H201514)。

摘　　要：目的了解南京地区汉族血小板捐献人群血小板抗原1~29(HPA-1-29bw)等位基因频率,为患者相容性血小板输注提供依据。方法采用一代测序法(Sanger测序法)对2019年2月~2019年9月在本中心捐献血小板的900名南京汉族固定捐献者做HPA基因分型,并采用直接计数法计算等位基因频率和基因型频率等。结果本组900名汉族单采血小板捐献者HPA等位基因频率分别为:HPA-1a 0.995 0、1b 0.005 0,2a 0.9467、2b 0.053 3,3a 0.585 0、3b 0.415 0,4a 0.998 9、4b 0.001 1,5a 0.982 2、5b 0.017 8,6a 0.982 8、6b 0.017 2,11a 0.999 4、11b 0.000 6,15a 0.531 7、15b 0.468 3,21a 0.992 8、21b 0.007 2;HPA-7-10w、-12-14w、-16-20w和-22-29bw均只检出单一a等位基因;本组中HPA-15不配合率最高[37.40%(337/900)],其次为HPA-3[36.77%(331/900)],另外在HPA-11w中检出1例杂合子。结论南京地区汉族血小板捐献人群HPA等位基因频率特点是HPA-15和HPA-3为最常见的杂合子,在本地临床输注中须引起重视。Objective To analyze the allele frequencies of the human platelet antigens 1-29 system(HPA-1-29bw) in Nanjing Han platelet donors, so as to provide references for compatible platelet transfusion. Methods HPA genotyping was performed by Sanger sequencing method in 900 Nanjing Han regular platelet donors who donated at Jiangsu Province Blood Center from February to September 2019. The frequencies of alleles and genotype were calculated using direct counting method. Results The HPA allele frequencies in Nanjing Han platelet donors were HPA-1a 0.9950, 1b 0.0050, 2a 0.9467, 2b 0.0533, 3a 0.5850, 3b 0.4150, 4a 0.9989, 4b 0.0011, 5a 0.9822, 5b 0.0178, 6a 0.9828, 6b 0.0172, 11a 0.9994, 11b 0.0006, 15a 0.5317, 15b 0.4683, 21a 0.9928 and 21b 0.0072, respectively. Only a allele was detected in HPA-7-10w,-12-14w,-16-20w and-22-29bw systems.The highest mismatch rate of HPA genes in 900 platelet donors was HPA-15 system, followed by HPA-3 system, with the rate of 37.40%(337/900) and 36.77%(331/900), respectively. One heterozygote was detected in HPA-11w system. Conclusion The chracteristics of HPA alleles frequencies in Nanjing Han platelet donors is that HPA-15 and HPA-3 are the most common heterozygotes, which should be paid attention to in local clinical transfusion.

关 键 词：血小板抗原 HPA基因 基因频率 血小板捐献者 

分 类 号：R457.11[医药卫生—治疗学] Q343.1[医药卫生—临床医学]