中亚苦蒿提取物对树突状细胞成熟及功能的影响  

Effect of Artemisia absinthium L.extract on the maturation and function of dendritic cells

作　　者：陈秋艳 夏丽洁[1] 迪丽尼格尔·孜亚依丁 徐朝霞 韩鹏 李金耀[1] Chen Qiuyan;Xia Lijie;Ziyayiding Dilinigeer;Xu Zhaoxia;Han Peng;Li Jinyao(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China;Urumqi No.23 Middle School,Urumqi 830000,China)

机构地区：[1]新疆大学生命科学与技术学院,生物资源基因工程重点实验室,乌鲁木齐830046 [2]乌鲁木齐市第二十三中学,830000

出　　处：《国际生物医学工程杂志》2021年第1期1-11,共11页International Journal of Biomedical Engineering

基　　金：国家自然科学基金(31860258);“天山青年计划”青年博士科技人才项目(2017Q077)。

摘　　要：目的探讨中亚苦蒿硅胶柱分离组分(AEM-SC)对小鼠树突状细胞(DC)成熟及免疫功能的影响。方法制备中亚苦蒿大孔吸附树脂70%乙醇洗脱组分,经硅胶柱进一步分离得到洗脱组分AEM-SC,并对其多糖、总黄酮、三萜类含量进行测定。流式细胞术检测AEM-SC对DC表面分子表达水平、抗原吞噬能力及刺激同种异体T细胞增殖能力的影响;酶联免疫吸附测定(ELISA)法检测AEM-SC对DC细胞因子表达的影响。结果AEM-SC中多糖、黄酮类和萜类的含量分别为10.12%、5.70%和3.62%。体外功能实验结果显示,AEM-SC可明显降低脂多糖诱导的DC表面分子CD40、CD86和主要组织相容性复合体Ⅱ类(MHC-Ⅱ)的表达以及细胞因子白细胞介素-12p40(IL-12p40)、肿瘤坏死因子α(TNF-α)和IL-6的水平(均P<0.05),提高DC摄取抗原的能力(P<0.01),降低DC刺激小鼠脾脏CD4+T与CD8+T淋巴细胞增殖的能力(均P<0.001)。小鼠炎症模型实验中,AEM-SC可明显降低脂多糖诱导的小鼠体内DC表面分子CD40、CD86、CD80的表达(均P<0.001)以及血清中细胞因子TNF-α、IL-12p40的表达(均P<0.01)。结论AEM-SC在体外与体内实验中均表现出抑制DC成熟的能力,显示AEM-SC具有一定的免疫抑制作用。Objective To investigate the effect of silica gel column separation component of Artemisia asiatica(AEM-SC)on the maturation and immune function of mouse dendritic cells(DCs).Methods Artemisia asiatica components were prepared by macroporous resin eluted with 70%ethanol,and then isolated by silica gel column to obtain AEM-SC.The contents of polysaccharides,flavonoids and triterpenes were quantified.Flow cytometry was used to detect the expression level of DCs surface molecules and antigen phagocytosis ability and to stimulate the proliferation of allogeneic T cells.ELISA method was used to detect the effect of DCs on cytokine secretion.Results The contents of polysaccharides,flavonoids and triterpenes in AEM-SC were 10.12%,5.7%and 3.62%,respectively.Functional tests showed that AEM-SC significantly reduced the expression levels of LPS-induced DCs surface molecules CD40,CD86 and MHC-II,reduced the expression levels of inflammatory cytokines IL-12p40,TNF-αand IL-6(all P<0.05),improve the ability of phygocytosis(P<0.01),and reduce the ability of DCs to stimulate the proliferation of CD4+T and CD8+T lymphocytes in the spleen of mice(all P<0.001).In the inflammatory mouse model experiment,AEM-SC significantly reduced the expression levels of DCs surface molecules CD40,CD86,CD80(all P<0.001),and the expression levels of inflammatory cytokines TNF-α,IL-12p40 in serum(all P<0.01).Conclusions AEM-SC can inhibit the maturation of DCs-induced LPS both in vitro and in vivo,indicating that AEM-SC has the immunosuppressive effect.

关 键 词：细胞因子 树突状细胞 中亚苦蒿 免疫抑制 

分 类 号：R285.5[医药卫生—中药学]