金钱草煎汤炒炙雷公藤的炮制减毒工艺研究  

Processing technology for reducing toxicity of fried Tripterygium wilfordii in Lysimachia christinae decoction

作　　者：关月晨 王君明[1,2] 宋玲玲 巫晓慧 刘晨 薛洁 GUAN Yue-chen;WANG Jun-ming;SONG Ling-ling;WU Xiao-hui;LIU Chen;XUE Jie(College of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan&Education Ministry of P.R.China,Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南中医药大学呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046

出　　处：《中国中药杂志》2021年第5期1128-1133,共6页China Journal of Chinese Materia Medica

基　　金：河南省高校科技创新人才支持计划项目(16HASTIT032);河南中医药大学科技创新人才支持计划项目(2015XCXRC01)。

摘　　要：该文主要优选金钱草煎汤炙雷公藤的炮制减毒工艺。以金钱草质量分数、闷润时间、炒炙时间、炒炙温度,按照4因素3水平正交试验设计表制备金钱草煎汤炙雷公藤炮制品共9个,HPLC测定炮制前后雷公藤中主成分雷公藤甲素(triptolide)的质量分数分别为4.27,3.92,3.57,2.75,2.42,2.66,3.51,1.87,1.75,2.03μg·g^(-1)。在此基础上,将以上各炮制品分别口服给予小鼠28 d,末次给药后12 h禁食不禁水,24 h后摘眼球取血清和肝组织,依据试剂盒方法检测血清生化指标以及肝组织脂质过氧化和抗氧化相关指标。雷公藤生品口服给予28 d后,诱导了小鼠的血清肝功指标谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱性磷酸酶(ALP)以及肝脂质过氧化产物(MDA)的水平分别显著升高了91%(P<0.01),46%(P<0.05),73%(P<0.01)和99%(P<0.01),肝抗氧化指标超氧化物歧化酶(T-SOD)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GPX)和谷胱甘肽-S转移酶(GST)的水平均显著降低(P<0.01);而各炮制品给药后,以上指标的异常均被显著逆转(P<0.01或P<0.05),其中尤以在因素水平为A_(3)B_(2)C_(1)D_(3)的工艺条件下对雷公藤的炮制减毒作用最佳,能使雷公藤引起的过高的AST,ALT,ALP,MDA水平分别降低49%(P<0.01),32%(P<0.01),42%(P<0.01),17%(P<0.05)。因此,金钱草煎汤炙雷公藤炮制减毒的最佳工艺条件为A3B2C1D3,即"金钱草质量分数15%,闷润时间1 h,炒炙温度160℃,炒炙时间9 min"。On the basis of the previous work of the research group,the orthogonal design method was further used to optimize the processing technology for reducing toxicity of fried Tripterygium wilfordii in Lysimachia christinae Decoction.A total of 9 processed products of T.wilfordii in L.christinae decoction were prepared by four factors and three levels orthogonal design table.The contents of triptolide in T.wilfordii were determined by high performance liquid chromatography(HPLC)before and after processing:4.27,3.92,3.57,2.75,2.42,2.66,3.51,1.87,1.75,2.03μg·g^(-1).On this basis,the above processed products were orally given to mice for 28 days.12 hours after the last administration,food fasting except water was provided,and 24 hours later,the eyeballs were taken for blood and liver tissue.Serum biochemical indexes,liver lipid peroxidation and antioxidant related indexes were detected by kit method.Twenty-eight days after oral administration of raw T.wilfordii,the levels of serum alanine aminotransferase(AST),alanine aminotransferase(ALT),alkaline phosphatase(ALP)and liver malondialdehyde(MDA)in mice increased by 91%(P<0.01),46%(P<0.05),73%(P<0.01)and 99%(P<0.01),while the liver antioxidant indexes such as superoxide dismutase(SOD),glutathione(GSH),glutathione peroxidase(GPX)and glutathione-S transferase(GST)significantly decreased(P<0.01).After administration of the processed products,the above indexes were significantly reversed(P<0.01 or P<0.05).Especially,the processing conditions of A_(3)B_(2)C_(1)D_(3)had the best detoxification effect on T.wilfordii,which decreased the high levels of AST,ALT,ALP and MDA by 49%(P<0.01),32%(P<0.01),42%(P<0.01),and 17%(P<0.05).Therefore,the best processing conditions for T.wilfordii in L.christinae decoction were A3B2C1D3,namely"15%mass fraction of L.christinae,1 h moistening time,160℃frying temperature,and 9 min frying time".

关 键 词：雷公藤 金钱草煎汤炙雷公藤 正交试验设计 炮制减毒工艺 氧化/抗氧化失衡 

分 类 号：R283.3[医药卫生—中药学]