不同浓度富血小板血浆修复兔膝骨关节炎软骨缺损的比较  

Different concentrations of platelet rich plasma in the repair of cartilage defects in rabbits with knee osteoarthritis

作　　者：房昕[1] 郭金铭 刘品端 Fang Xin;Guo Jinming;Liu Pinduan(Department of Orthopedics,Tieling Central Hospital,Tieling 112000,Liaoning Province,China;Department of Orthopedics,Shengjing Hospital Affiliated to China Medical University,Shenyang 110000,Liaoning Province,China;Department of Orthopedics,the First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,Liaoning Province,China)

机构地区：[1]铁岭市中心医院骨科,辽宁省铁岭市112000 [2]中国医科大学附属盛京医院骨科,辽宁省沈阳市110000 [3]锦州医科大学附属第一医院骨科,辽宁省锦州市121000

出　　处：《中国组织工程研究》2021年第35期5588-5593,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:体内和体外的研究均表明,富血小板血浆对促进软骨细胞增殖和组织再生修复起重要作用,但对于不同浓度富血小板血浆修复兔膝骨关节炎软骨缺损的研究较少。目的:比较不同浓度富血小板血浆修复兔膝骨关节炎软骨缺损的效果,为临床治疗膝骨关节炎软骨损伤提供理论依据和选择方案。方法:50只健康新西兰大白兔随机被分为空白组、模型组、富血小板血浆低浓度、中浓度、高浓度组,每组10只。二次离心法制备富血小板血浆。除空白组以外,各组采用改良Hulth法制备兔膝骨关节炎模型。模型组在关节腔注射0.3 mL生理盐水,富血小板血浆低浓度、中浓度、高浓度组分别注射浓度为(1.0-1.2)×10^(12)L-1、(1.5-1.7)×10^(12)L-1、(2.0-2.2)×10^(12)L-1的富血小板血浆。采用ELISA法检测关节液中相关炎症因子肿瘤坏死因子α、白细胞介素1β和白细胞介素6的表达;苏木精-伊红染色、甲苯胺蓝染色和Mankin’s病理评分来评估不同浓度富血小板血浆修复膝骨关节炎软骨缺损后的组织学变化;Western blot和RT-PCR检测关节软骨组织中Ⅱ型胶原蛋白和聚集蛋白聚糖的蛋白及mRNA表达水平。实验方案经中国医科大学动物实验动物福利与伦理委员会批准。结果与结论:①与模型组比较,富血小板血浆各浓度组相关炎症因子肿瘤坏死因子α、白细胞介素1β和白细胞介素6的水平均有所下降(P<0.05),中浓度组表达水平少于低浓度组和高浓度组(P<0.05);②苏木精-伊红和甲苯胺蓝染色结果显示,富血小板血浆低浓度组和高浓度组软骨细胞形态较规则,数量有所增加,中浓度组软骨细胞排列较整齐,有软骨样细胞形成,软骨细胞修复效果明显;③Mankin’s病理评分结果,模型组最高,其次富血小板血浆低浓度和高浓度组,中浓度组评分最低;④Western blot和RT-PCR结果显示,与模型组比较,富血小板血浆各浓度组软骨组BACKGROUND:Both in vivo and in vitro studies have shown that platelet-rich plasma plays an important role in promoting chondrocyte proliferation and tissue regeneration and repair.However,little is reported on the repair of cartilage defects with different concentrations of platelet-rich plasma in rabbits with knee osteoarthritis.OBJECTIVE:To compare the different concentrations of platelet rich plasma in the repair of cartilage defects in rabbits with knee osteoarthritis,so as to provide theoretical basis and options for the clinical treatment of cartilage defects in knee osteoarthritis.METHODS:A total of 50 healthy New Zealand white rabbits were randomly divided into blank group,model group,low concentration,medium concentration and high concentration groups of platelet-rich plasma with 10 rabbits in each group.Platelet-rich plasma was prepared by secondary centrifugation.An osteoarthritis model of rabbit knee was made by improved Hulth method in each group except for the blank group.In the model group,0.3 mL of normal saline was injected into the articular cavity,while in the low,medium and high concentration groups,(1.0-1.2)×10^(12)/L,(1.5-1.7)×10^(12)/L,and(2.0-2.2)×10^(12)/L platelet-rich plasma was injected respectively.The expression of tumor necrosis factor-α,interleukin-1βand interleukin-6 in the synovial fluid was detected by ELISA,and the histological changes of different concentrations of platelet-rich plasma were evaluated by hematoxylin-eosin staining,toluidine blue staining and Mankin’s pathological score.Western blot and RT-PCR were used to detect the protein and mRNA levels of type Ⅱ collagen and aggrecan.The study protocol was approved by the Experimental Animal Ethics Committee of China Medical University.RESULTS AND CONCLUSION:Compared with the model group,the levels of tumor necrosis factor-α,interleukin-1βand interleukin-6 in the low,middle and high concentration groups decreased to some extent(P<0.05),the expression levels of these indicators in the middle concentration group we

关 键 词：富血小板血浆 骨关节炎 软骨缺损 炎症因子 Ⅱ型胶原蛋白 聚集蛋白聚糖 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]